Success of embryo transfer in mice with freshly collected and cryopreserved two-cell embryos with different genetic backgrounds correlated with the number of transferred embryos: A 5-year retrospective analysis
| Autor: | Wiebke Garrels, Isabell Wittur, Martina Dorsch |
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| Rok vydání: | 2019 |
| Předmět: |
animal structures
medicine.medical_treatment Cell Embryonic Development Biology Cryopreservation Andrology 03 medical and health sciences Mice 0302 clinical medicine Pregnancy Retrospective analysis medicine Animals 030304 developmental biology Retrospective Studies 0303 health sciences Mice Inbred BALB C 030219 obstetrics & reproductive medicine In vitro fertilisation General Veterinary Embryo Embryo Transfer Embryo Mammalian Embryo transfer Mice Inbred C57BL medicine.anatomical_structure embryonic structures Animal Science and Zoology Female |
| Zdroj: | Laboratory animals. 53(6) |
| ISSN: | 1758-1117 |
| Popis: | Embryo transfer of pre-implantation embryos to surrogate dams is a key technique for the hygienic sanitation of strains, cryopreservation, in vitro fertilization, genetic modification and engineering. However, the effects of several parameters, such as the number of transferred embryos, on the success of embryo transfer are not well studied. In this retrospective study, we reanalysed 1320 embryo transfers of two-cell embryos originating from genetically altered donors, which were performed under routine conditions in our facility over a period of 5 years. Of them, 453 embryo transfers were done with freshly collected embryos and 867 transfers were performed with cryopreserved embryos. Despite the fact that the genetic background of the embryo donors was quite heterogeneous, we found that the transfer of ≥ 21 embryos reduced the success of embryo transfers for freshly collected embryos in correlation with the number of pregnancies and born pups, whereas this was not the case for transfer in the cryopreservation group. Most pregnancies were achieved after embryo transfer of 10–20 freshly collected embryos (90.4%), which dropped to 37.5% if more embryos were transferred. The highest pregnancy rates in the cryopreservation group were achieved if 15–17 embryos were transferred (62.9%). Despite the fact that the precise substrains were only rarely defined, we confirmed that beside the number of transferred embryos, the genetic background of the donors had an influence on the success of embryo transfer. Significantly more embryos in a C57BL/6 background developed to term than embryos on a BALB/c background. |
| Databáze: | OpenAIRE |
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