Immunogenicity analysis of decellularized cardiac scaffolds after transplantation into rats
| Autor: | Cheng Li, Feifei Du, Cailing Tong, Zhonggui Shan, Minghui Li, Junjie Xia, Zhongquan Qi, Yaguang Li, Baiyi Xie |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Graft Rejection
Embryology medicine.medical_specialty 0206 medical engineering Biomedical Engineering Hamster 02 engineering and technology Pharmacology Organ transplantation Extracellular matrix 03 medical and health sciences Tissue engineering Cricetinae medicine Animals Autografts 030304 developmental biology 0303 health sciences Decellularization Mesocricetus Tissue Scaffolds Chemistry Myocardium Immunogenicity Allografts 020601 biomedical engineering Extracellular Matrix Rats Transplantation Artificial organ Rats Inbred Lew Heterografts Female |
| Zdroj: | Regenerative Medicine. 14:447-464 |
| ISSN: | 1746-076X 1746-0751 |
| DOI: | 10.2217/rme-2018-0139 |
| Popis: | Aim: Cardiac extracellular matrix (cECM) scaffolds are promising biomaterials for clinical applications. Our aim is to determine the immunogenicity of decellularized scaffolds from different sources for use as artificial organs during organ transplantation. Materials & methods: We transplanted Lewis rats with syngeneic (Lewis rat cECM), allogeneic (BN rat cECM) or xenogeneic (hamster cECM) decellularized cardiac scaffolds. Acute vascular and cellular rejection was quantified by immunohistochemistry and immune cell infiltration. Results: BN rat and hamster hearts were rejected following transplantation. BN and hamster cECMs had similarly low immunogenicity compared with Lewis rat cECMs and did not lead to increased rejection. Conclusion: We found that scaffolds from all sources did not induce vascular or cellular rejection and exhibited low immunogenicity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|