Knockdown of inhibitor of differentiation 1 suppresses proliferation and induces apoptosis by inactivating PI3K/Akt/mTOR signaling in hemangioma-derived endothelial cells
Autor: | Miaomiao Li, Longlong Sun, Bin Sun, Yuanfang Zhang, Hongyu Zhang, Hongzhao Lei, Yubin Gong, Changxian Dong |
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Rok vydání: | 2019 |
Předmět: |
Inhibitor of Differentiation Protein 1
0301 basic medicine Proliferation Apoptosis RM1-950 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Human Umbilical Vein Endothelial Cells Humans LY294002 Phosphatidylinositol Protein kinase A Protein kinase B PI3K/AKT/mTOR pathway Cell Proliferation Phosphoinositide-3 Kinase Inhibitors Pharmacology Gene knockdown Oncogene Chemistry TOR Serine-Threonine Kinases Endothelial Cells General Medicine 030104 developmental biology Gene Knockdown Techniques 030220 oncology & carcinogenesis Cancer research Therapeutics. Pharmacology Phosphatidylinositol 3-Kinase PI3K/Akt/mTOR Hemangioma ID-1 Proto-Oncogene Proteins c-akt Signal Transduction |
Zdroj: | Biomedicine & Pharmacotherapy, Vol 111, Iss, Pp 236-243 (2019) |
ISSN: | 0753-3322 |
Popis: | Hemangioma (HA) is one of the commonest benign vascular neoplasms of infancy. Inhibitor of differentiation 1 (ID-1) has been reported to be an oncogene in multiple cancers. However, the role of ID-1 and its molecular mechanism in HA progression have not been elucidated. In the present study, we found that ID-1 expression at mRNA and protein levels was up-regulated in HA-derived endothelial cells (HDECs). Knockdown of ID-1 inhibited proliferation, facilitated apoptosis, and enhanced propranolol cytotoxicity in HDECs. Knockdown of ID-1 decreased the protein levels of phospholyrated protein kinase-B (Akt) and phospholyrated mammalian target of rapamycin (mTOR). Inhibition of PI3K/Akt/mTOR pathway by LY294002 abrogated ID-1-mediated pro-proliferation and anti-apoptosis effects in HDECs. In conclusion, knockdown of ID-1 suppressed proliferation and promoted apoptosis by inactivating phosphatidylinositol 3-kinase (PI3K)/Akt/mTOR signaling in HDECs, shedding light on the function of ID-1 in HA progression and highlighting the therapeutic value of ID-1 for HA. |
Databáze: | OpenAIRE |
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