Polyelectrolyte multilayer films as substrates for photoreceptor cells
| Autor: | José-Alain Sahel, Fouzia Boulmedais, Pierre Schaaf, Aysen Tezcaner, David Hicks, Jean-Claude Voegel, Philippe Lavalle |
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| Přispěvatelé: | Department of Engineering Sciences, Middle East Technical University [Ankara] (METU), Laboratoire de Physiopathologie Cellulaire et Moleculaire de la Retine, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Biomatériaux : Processus biophysiques et biologiques aux interfaces, Université Louis Pasteur - Strasbourg I-Faculté de Chirurgie Dentaire-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de la Vision, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC), Institut Charles Sadron (ICS), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Matériaux et nanosciences d'Alsace (FMNGE), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), OpenMETU, Lavalle, Philippe, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Polypeptide Films
Chemical Phenomena Polymers and Plastics Survival Swine MESH: Chondroitin Sulfates Neurotrophic Factor Aucun 02 engineering and technology Microscopy Atomic Force 01 natural sciences Substrate Specificity law.invention Electrolytes chemistry.chemical_compound MESH: Electrolytes law Materials Chemistry Polylysine MESH: Animals Hyaluronic Acid MESH: Swine Cells Cultured MESH: Microscopy Atomic Force Chemistry Physical Atomic force microscopy Chondroitin Sulfates MESH: Polystyrenes Cell Differentiation Basic Fibroblast 021001 nanoscience & nanotechnology Polyelectrolyte Fibroblast-Growth-Factor MESH: Cell Survival Fibroblast Growth Factor 2 0210 nano-technology Layer (electronics) MESH: Cells Cultured MESH: Cell Differentiation Materials science MESH: Microscopy Electron Scanning Cell Survival Bioengineering chemistry 010402 general chemistry Biomaterials cytology drug effects metabolism Confocal microscopy Polymer chemistry Animals Photoreceptor Cells MESH: Chemistry Physical Chondroitin sulfate Spectroscopy By-Layer Deposition [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials MESH: Photoreceptors Interphotoreceptor Matrix MESH: Fibroblast Growth Factor 2 MESH: Hyaluronic Acid Rod Photoreceptors ultrastructure Quartz crystal microbalance MESH: Polylysine 0104 chemical sciences [SDV.IB.BIO] Life Sciences [q-bio]/Bioengineering/Biomaterials Chemical engineering Microscopy Electron Scanning Polystyrenes Rat MESH: Substrate Specificity Self-assembly sense organs pharmacology Bioactive Coatings |
| Zdroj: | Biomacromolecules Biomacromolecules, American Chemical Society, 2006, 7 (1), pp.86-94. ⟨10.1021/bm0505134⟩ Biomacromolecules, 2006, 7 (1), pp.86-94. ⟨10.1021/bm0505134⟩ |
| ISSN: | 1525-7797 1526-4602 |
| DOI: | 10.1021/bm0505134⟩ |
| Popis: | Reconstruction of extracellular matrix substrates for delivery of functional photoreceptors is crucial in pathologies such as retinal degeneration and age-related macular degeneration. In this study, we assembled polyelectrolyte films using the layer-by-layer deposition method. The buildup of three different films composed of poly(L-lysine)/chondroitin sulfate (PLL/CSA), poly(L-lysine)/poly(styrenesulfonate) (PLL/PSS), or poly(L-lysine)/hyaluronic acid (PLL/HA) was followed by means of quartz crystal microbalance measurements, optical waveguide light mode spectroscopy, confocal microscopy, and atomic force microscopy. The exponential growth regime and the diffusion of PLL chains from the bulk through the PLL/CSA, PLL/PSS, and PLL/HA films was examined. Evaluation of photoreceptor cell viability was optimal on one layer of PLL (PLL(1)), followed by 10 bilayers of PLL/HA [(PLL/HA)(10)] and 10 bilayers of PLL/CSA [(PLL/CSA)(10)]. The number of bilayers and the type of terminating layer also had a significant influence on the number of photoreceptor cells attached. Functionalized polyelectrolyte multilayer films were obtained by adsorbing basic fibroblastic factor (bFGF) or the insoluble fraction of interphotoreceptor matrix (IPM) on or within polyelectrolyte multilayers. bFGF and IPM adsorption on top of the (PLL/CSA)(10)/PLL polyelectrolyte films increased the number of photoreceptor cells attached and maintained the differentiation of rod and cone cells. journal article research support, non-u.s. gov't 2006 Jan imported |
| Databáze: | OpenAIRE |
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