mGluR-mediated and endocannabinoid-dependent long-term depression in the hilar region of the rat dentate gyrus
| Autor: | Casie Lindsly, Ben Nahir, Charles J. Frazier |
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| Rok vydání: | 2009 |
| Předmět: |
Agonist
Male medicine.drug_class Mice Transgenic Neurotransmission Inhibitory postsynaptic potential Receptors Metabotropic Glutamate Article Methoxyhydroxyphenylglycol Rats Sprague-Dawley Cellular and Molecular Neuroscience Mice Receptor Cannabinoid CB1 Cannabinoid Receptor Modulators medicine Animals Long-term depression Pharmacology Mice Knockout Chemistry Dentate gyrus Long-Term Synaptic Depression Endocannabinoid system Rats Mice Inbred C57BL nervous system Inhibitory Postsynaptic Potentials Metabotropic glutamate receptor Synaptic plasticity Dentate Gyrus Neuroscience Endocannabinoids |
| Zdroj: | Neuropharmacology. 58(4-5) |
| ISSN: | 1873-7064 |
| Popis: | We report that bath application of the group I mGluR agonist (RS)-3,5-dihydroxyphenylglycine (DHPG) causes acute inhibition of evoked IPSCs recorded from hilar mossy cells, and that significant long-term depression (LTD) of synaptic transmission remains following washout of DHPG. Subsequent experiments using minimal stimulation techniques revealed that expression of both acute and long-term effects of DHPG are restricted to a subset of GABAergic afferents that are also sensitive to depolarization-induced suppression of inhibition (DSI). Experiments with a selective CB1 antagonist and with transgenic animals lacking CB1 receptors indicate that all effects of DHPG, like DSI, depend on activation of CB1 receptors. Further work with selective mGluR antagonists suggests a direct involvement of mGluR1 receptors. Interestingly, we also report that induction of LTD under our experimental conditions does not require prior direct somatic depolarization via the patch pipette and does not appear to depend critically on the level of activity in incoming GABAergic afferents. Collectively, these results represent the first characterization of mGluR-mediated and endocannabinoid-dependent LTD in the hilar region of the dentate gyrus. The dentate gyrus is thus one of relatively few areas where this mechanism has clearly been demonstrated to induce long-term modulation of inhibitory synaptic transmission. |
| Databáze: | OpenAIRE |
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