Dysregulation of IL-2 and IL-8 production in circulating T lymphocytes from young cystic fibrosis patients

Autor: Dominique Gaillard, C. Hubeau, R. Le Naour, Moncef Guenounou, Jocelyne Hinnrasky, M. Abely, E. Puchelle
Přispěvatelé: Dynamique cellulaire et moléculaire de la muqueuse respiratoire, Université de Reims Champagne-Ardenne (URCA)-IFR53-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire d'Immunologie, Virologie et Bactériologie, IFR53, Université de Reims Champagne-Ardenne (URCA), Birembaut, Philippe
Jazyk: angličtina
Rok vydání: 2004
Předmět:
Interleukin 2
medicine.medical_specialty
MESH: Cystic Fibrosis
MESH: Antigens
CD3
CD3
medicine.medical_treatment
Immunology
MESH: Cytopla
MESH: Flow Cytometry
MESH: T-Lymphocyte Subsets
medicine.disease_cause
[SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract
03 medical and health sciences
0302 clinical medicine
Internal medicine
Immunopathology
MESH: Child
Clinical Studies
medicine
Immunology and Allergy
Interleukin 8
MESH: Lymphocyte Activation
030304 developmental biology
MESH: Adolescent
0303 health sciences
MESH: Cytokines
MESH: Humans
biology
business.industry
MESH: Cytoplasm
Interleukin
MESH: Interleukin-2
T lymphocyte
Immune dysregulation
MESH: Male
3. Good health
MESH: Interleukin-8
Endocrinology
Cytokine
030228 respiratory system
biology.protein
[SDV.MHEP.PSR] Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract
business
MESH: Female
medicine.drug
Zdroj: Clinical and Experimental Immunology
Clinical and Experimental Immunology, Wiley, 2004, 135 (3), pp.528-34. ⟨10.1111/j.1365-2249.2003.02385.x⟩
ISSN: 0009-9104
1365-2249
DOI: 10.1111/j.1365-2249.2003.02385.x⟩
Popis: SUMMARY It is well documented that patients with cystic fibrosis (CF) are unable to clear persistent airway infections in spite of strong local inflammation, suggesting a dysregulation of immunity in CF. We and others have reported previously that T lymphocytes may play a prominent role in this immune imbalance. In the present work, we compared the reactivity of CD3+ T cells obtained from young CF patients in stable clinical conditions (n = 10, aged 9–16·5 years) to age-matched healthy subjects (n = 6, aged 9–13·5 years). Intracellular levels of interferon (IFN)-γ, interleukin (IL)-2, IL-8 and IL-10 were determined by flow cytometry after whole blood culture. The data identified T lymphocyte subsets producing either low levels (M1) or high levels (M2) of cytokine under steady-state conditions. We found that the production of IFN-γ and IL-10 by T lymphocytes was similar between young CF patients and healthy subjects. In contrast, after 4 h of activation with PMA and ionomycin, the percentage of T cells producing high levels of IL-2 (M2) was greater in CF patients (P = 0·02). Moreover, T cells from CF patients produced lower levels of IL-8, before and after activation (P = 0·007). We conclude that a systemic immune imbalance is present in young CF patients, even when clinically stable. This disorder is characterized by the capability of circulating T lymphocytes to produce low levels of IL-8 and by the emergence of more numerous T cells producing high levels of IL-2. This imbalance may contribute to immune dysregulation in CF.
Databáze: OpenAIRE
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