Antimikrobno i antiproliferativno djelovanje derivata ksanten-3-ona i njihov afinitet vezivanja za enzime
| Autor: | Elma Veljović, Anja Harej, Marko Klobučar, Sandra Kraljević Pavelić, Selma Špirtović-Halilović, Amar Osmanović, Samija Muratović, Mirsada Salihović, Davorka Završnik |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
General Chemical Engineering
ksanteni antibakterijska i antifungalna aktivnost indukcija stanične smrti načini vezanja za enzime docking studija predviđanje bioraspoloživosti General Chemistry xanthenes antibacterial and antifungal activity induction of cell death enzyme binding modes docking study bioavailability prediction |
| Zdroj: | Kemija u industriji : Časopis kemičara i kemijskih inženjera Hrvatske Volume 71 Issue 3-4 |
| ISSN: | 1334-9090 0022-9830 |
| Popis: | Ten biologically active derivatives of 2,6,7-trihydroxyxanthen-3-one, previously synthesized and characterised, were investigated for their in vitro antimicrobial and antiproliferative activity. Compounds were tested on three bacteria, Staphylococcus aureus, Bacillus subtilis and Escherichia coli, and two fungi strains, Candida albicans, and Saccharomyces cerevisiae. The best activity against E. coli showed non-substituted compound 1. The most potent against fungi strains was compound 7 with ortho methoxy substituent. Compound 4 exerted the most potent antiproliferative activity in the micromolar range (0.1–10 µM) on tested tumour cell lines except on SW620. Additional Western blot analyses showed increased cyclin B1 levels in HeLa cells treated with compound 4, which is a major mitotic catastrophe’s marker and decreased levels of Wee1 and Erk ½ kinases involved in regulation of the mitotic process. The most potent compounds after in vitro tests were subjected to molecular docking simulations to evaluate enzyme binding affinity, and provide further evidence for experimentally observed biological effects in vitro. This work is licensed under a Creative Commons Attribution 4.0 International License. Za deset prethodno sintetiziranih biološki aktivnih derivata 2,6,7-trihidroksiksanten-3-ona ispitivano je in vitro antimikrobno i antiproliferativno djelovanje. Mikrobiološka aktivnost spojeva ispitana je na tri bakterije: Staphylococcus aureus, Bacillus subtilis i Escherichia coli, te dva soja gljiva: Candida albicans i Saccharomyces cerevisiae. Najbolju antibakterijsku aktivnost protiv E. coli pokazao je nesupstituirani spoj 1. Najveću antifungalnu aktivnost pokazao je spoj 7 s orto metoksi supstituentom. Spoj 4 pokazao je najjače antiproliferativno djelovanje u mikromolarnom rasponu (0,1 – 10 µM) na testiranim linijama tumorskih stanica, osim na SW620. Dodatne Western blot analize pokazale su povišenu razinu ciklina B1 u HeLa stanicama tretiranim spojem 4, koji je glavni marker mitotske katastrofe, i smanjenu razinu Wee1 i Erk ½ kinaza uključenih u regulaciju mitotskog procesa. Najpotentniji spojevi podvrgnuti su molekularnim docking simulacijama da bi se procijenio afinitet vezanja za enzime i pružili daljnji dokazi o eksperimentalno opaženim biološkim učincima in vitro. Ovo djelo je dano na korištenje pod licencom Creative Commons Imenovanje 4.0 međunarodna. |
| Databáze: | OpenAIRE |
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