Regulatory mechanisms for natriuretic peptide signalling in sheep granulosa cells
| Autor: | Peter Marsters, Rana Alhamdan, Juan H. Hernandez-Medrano, Walid E. Maalouf, Bruce K. Campbell |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Vascular Endothelial Growth Factor A
medicine.medical_specialty medicine.drug_class Cellular differentiation Proximity ligation assay Paracrine signalling Endocrinology Internal medicine Gene expression Natriuretic peptide medicine Animals Natriuretic Peptides Receptor Autocrine signalling Molecular Biology Cells Cultured Progesterone Granulosa Cells Sheep Estradiol Chemistry Natriuretic Peptide C-Type Gene Expression Regulation Theca Female Atrial Natriuretic Factor hormones hormone substitutes and hormone antagonists Signal Transduction |
| Zdroj: | Journal of Molecular Endocrinology. 64:223-234 |
| ISSN: | 1479-6813 0952-5041 |
| Popis: | Natriuretic peptides (NPs) have been reported to have critical roles in follicular development and oocyte maturation in rodents. This study aimed to extend our current understanding of NP-mediated signalling pathways and mechanisms of action in the follicles of a monovulatory species. Ovine granulosa cells (GCs) and theca cells (TCs) were cultured under conditions designed to allow gonadotrophin-stimulated cell differentiation. Gene expression analysis was performed by qualitative (q)PCR for NPs and NPRs (between 16 and 96 h of culture) and VEGF120 and VEGF164 (between 16 and 144 h of culture). A qualitative analysis of the production of NP/NPR family members and NP ligand/receptor associations was carried out utilising a highly sensitive immunological approach known as ‘proximity ligation assay’ (PLA). All NPRs were observed in GCs, while NPRA was absent in TCs. In GCs, gene expression of NPRA, NPRB and NPRC was apparent but only active BNP and CNP and not ANP, were detected. Also in GCs, ANP but not CNP was able to significantly (P P 164 by ANP and CNP (P 120 by ANP (P |
| Databáze: | OpenAIRE |
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