Effect of insulin–transferrin–selenium (ITS) andl -ascorbic acid (AA) duringin vitromaturation onin vitrobovine embryo development
| Autor: | Margot Alves Nunes Dode, Sidney A. Pereira, A. L. S. Guimarães, M. N. Diógenes |
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| Rok vydání: | 2016 |
| Předmět: |
Male
Ascorbic Acid Fertilization in Vitro Cleavage (embryo) Embryo Culture Techniques Andrology Selenium 03 medical and health sciences 0302 clinical medicine medicine Animals Insulin Blastocyst 030219 obstetrics & reproductive medicine Chemistry Embryogenesis Transferrin 0402 animal and dairy science Embryo 04 agricultural and veterinary sciences Cell Biology Ascorbic acid Oocyte 040201 dairy & animal science Culture Media In Vitro Oocyte Maturation Techniques In vitro maturation medicine.anatomical_structure embryonic structures Oocytes Cattle Female Embryo quality Developmental Biology |
| Zdroj: | Zygote. 24:890-899 |
| ISSN: | 1469-8730 0967-1994 |
| DOI: | 10.1017/s0967199416000228 |
| Popis: | SummaryThe aim of this study was to evaluate the effect of adding a combination of insulin, transferrin and selenium (ITS) andl-ascorbic acid (AA) duringin vitromaturation (IVM) andin vitroculture (IVC) onin vitroembryo production. To verify the effect of the supplements, cleavage and blastocyst rates, embryo size and total cell number were performed. Embryonic development data, embryo size categorization and kinetics of maturation were analyzed by chi-squared test, while the total cell number was analyzed by a Kruskal–Wallis test (P< 0.05). When ITS was present during IVM, IVC or the entire culture, all treatments had a cleavage and blastocyst rates and embryo quality, similar to those of the control group (P< 0.05). Supplementation of IVM medium with ITS and AA for 12 h or 24 h showed that the last 12 h increased embryo production (51.6%;n= 220) on D7 compared with the control (39.5%;n= 213). However, no improvement was observed in blastocyst rate when less competent oocytes, obtained from 1–3 mm follicles, were exposed to ITS + AA for the last 12 h of IVM, with a blastocyst rate of 14.9% (n= 47) compared with 61.0% (n= 141) in the control group. The results suggest that the addition of ITS alone did not affect embryo production; however, when combined with AA in the last 12 h of maturation, there was improvement in the quantity and quality of embryos produced. Furthermore, the use of ITS and AA during IVM did not improve the competence of oocytes obtained from small follicles. |
| Databáze: | OpenAIRE |
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