The atypical GATA protein TRPS1 represses androgen-induced prostate-specific antigen expression in LNCaP prostate cancer cells
| Autor: | Mila Jhamai, Gert-Jan C.M. van den Bemd, Glenn T.G. Chang, Albert O. Brinkmann |
|---|---|
| Přispěvatelé: | Developmental Biology |
| Rok vydání: | 2003 |
| Předmět: |
Male
Transcription Genetic GATA zinc finger Biophysics Down-Regulation Biology urologic and male genital diseases Biochemistry Prostate cancer SDG 3 - Good Health and Well-being Cell Line Tumor LNCaP medicine Humans Enhancer Molecular Biology GATA2 Nuclear Proteins Prostatic Neoplasms Cell Biology Metribolone Prostate-Specific Antigen medicine.disease Upstream Enhancer Neoplasm Proteins Androgen receptor DNA-Binding Proteins Gene Expression Regulation Neoplastic Repressor Proteins Cancer research Androgens GATA transcription factor Transcription Factors |
| Zdroj: | Biochemical & Biophysical Research Communications, 312, 578-584. Academic Press |
| ISSN: | 1090-2104 0006-291X |
| DOI: | 10.1016/j.bbrc.2003.10.154 |
| Popis: | Prostate-specific antigen (PSA) is considered as an important marker for prostate cancer. Regulation of PSA gene expression is mediated by androgens bound to androgen receptors via androgen response elements (AREs) in its promoter and far upstream enhancer regions. In addition, GATA proteins contribute to PSA gene transcription by interacting with GATA motifs present in the PSA enhancer sequence. The TRPS1 gene contains a single GATA zinc finger domain and not only binds to forward consensus GATA motifs but also to an inverse GATA motif overlapping the ARE III in the far upstream enhancer of the PSA gene. Overexpression of TRPS1 in androgen-dependent human LNCaP prostate cancer cells inhibited the transcription of a transiently transfected PSA enhancer/promoter-driven luciferase reporter construct. Furthermore, overexpression of TRPS1 reduced the androgen-induced endogenous PSA levels secreted in culture medium of LNCaP cells. Our results suggest a role of TRPS1 in androgen regulation of PSA gene expression. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect