Proton and phosphorus magnetic resonance spectroscopy of a mouse model of Alzheimer's disease
| Autor: | Vladimir Mlynarik, Lili Sun-Reimer, Cristina Cudalbu, Bernard L. Schneider, Patrick Aebischer, Hongxia Lei, Rolf Gruetter, Sharon Janssens, Matthias Cacquevel |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
In vivo magnetic resonance spectroscopy
Pathology Magnetic Resonance Spectroscopy Phosphocreatine Adenosine Triphosphate/metabolism Hippocampus Mice Adenosine Triphosphate Hippocampus/metabolism/pathology in vivo NMR spectroscopy Creatine Kinase Magnetic Resonance Spectroscopy/methods CIBM-AIT biology medicine.diagnostic_test General Neuroscience Brain Phosphorus General Medicine Nuclear magnetic resonance spectroscopy Alzheimer's disease 5xFAD metabolic profile Psychiatry and Mental health Clinical Psychology Aspartic Acid/analogs & derivatives/metabolism Protons Algorithms Alzheimer Disease/metabolism/pathology Genetically modified mouse medicine.medical_specialty Mice Transgenic transgenic mice ddc:616.0757 Brain Chemistry/physiology Neurochemical Alzheimer Disease In vivo medicine Animals Brain/pathology Pathological Protons/diagnostic use Brain Chemistry Aspartic Acid Phosphocreatine/metabolism Phosphorus/diagnostic use Magnetic resonance imaging Mice Inbred C57BL biology.protein Creatine kinase Geriatrics and Gerontology Creatine Kinase/metabolism Inositol/metabolism Inositol |
| Zdroj: | Journal of Alzheimer's Disease, Vol. 31 Suppl 3 (2012) pp. S87-99 |
| ISSN: | 1387-2877 |
| Popis: | The development of new diagnostic criteria for Alzheimer's disease (AD) requires new in vivo markers reflecting early pathological changes in the brain of patients. Magnetic resonance (MR) spectroscopy has been shown to provide useful information about the biochemical changes occurring in AD brain in vivo. The development of numerous transgenic mouse models of AD has facilitated the evaluation of early biomarkers, allowing researchers to perform longitudinal studies starting before the onset of the pathology. In addition, the recent development of high-field animal scanners enables the measurement of brain metabolites that cannot be reliably quantified at lower magnetic fields. In this report, we studied a new transgenic mouse model of AD, the 5xFAD model, by in vivo proton and phosphorus MR spectroscopy. This model, which is characterized by an early-onset and a robust amyloid pathology, developed changes in the neurochemical profile, which are typical in the human disease, i.e., an increase in myo-inositol and a decrease in N-acetylaspartate concentrations, as early as in the 40th week of age. In addition, a significant decrease in the γ-aminobutyrate concentration was observed in transgenic mice at this age compared to controls. The pseudo-first-order rate constant of the creatine kinase reaction as well as relative concentrations of phosphorus-containing metabolites were not changed significantly in the 36 and 72-week old transgenic mice. Overall, these results suggest that mitochondrial activity in the 5 × FAD mice is not substantially affected but that the model is relevant for studying early biomarkers of AD. |
| Databáze: | OpenAIRE |
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