The Convergence of Cell-Based Surface Plasmon Resonance and Biomaterials: The Future of Quantifying Bio-molecular Interactions—A Review
| Autor: | Jiaojiao Wang, Phillip Page, Spencer B. Mamer, Ali Ansari, Mary C. Murphy, P. I. Imoukhuede, Pierrick Gallerne |
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| Rok vydání: | 2019 |
| Předmět: |
Molecular interactions
Computer science Systems Biology Systems biology 0206 medical engineering Biomedical Engineering Biocompatible Materials Context (language use) Biosensing Techniques 02 engineering and technology Cells Immobilized Surface Plasmon Resonance Complex network 020601 biomedical engineering Article Kinetics Convergence (routing) Cell Adhesion Humans Biochemical engineering Surface plasmon resonance Cell based |
| Zdroj: | Ann Biomed Eng |
| ISSN: | 1573-9686 0090-6964 |
| Popis: | Cell biology is driven by complex networks of biomolecular interactions. Characterizing the kinetic and thermodynamic properties of these interactions is crucial to understanding their role in different physiological processes. Surface plasmon resonance (SPR)-based approaches have become a key tool in quantifying biomolecular interactions, however conventional approaches require isolating the interacting components from the cellular system. Cell-based SPR approaches have recently emerged, promising to enable precise measurements of biomolecular interactions within their normal biological context. Two major approaches have been developed, offering their own advantages and limitations. These approaches currently lack a systematic exploration of ‘best practices’ like those existing for traditional SPR experiments. Toward this end, we describe the two major approaches, and identify the experimental parameters that require exploration, and discuss the experimental considerations constraining the optimization of each. In particular, we discuss the requirements of future biomaterial development needed to advance the cell-based SPR technique. |
| Databáze: | OpenAIRE |
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