A CRISPR-Cas9 gene drive system targeting female reproduction in the malaria mosquito vector Anopheles gambiae
| Autor: | Roberto Galizi, Andrew Hammond, Alekos Simoni, Nikolai Windbichler, Austin Burt, Steven Russell, Eric Marois, Matthew Gribble, Dean A. Baker, Kyros Kyrou, Andrea Crisanti, Dimitris Katsanos, Tony Nolan, Carla Siniscalchi |
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| Přispěvatelé: | Grand Challenges in Global Health, The Royal Society, Commission of the European Communities, Galizi, Roberto [0000-0003-3134-7480], Windbichler, Nikolai [0000-0001-9896-1165], Apollo - University of Cambridge Repository |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
EXPRESSION
Animals Anopheles Female Malaria Clustered Regularly Interspaced Short Palindromic Repeats Insect Vectors 0301 basic medicine Anopheles gambiae Population Biomedical Engineering Bioengineering Locus (genetics) Engineered Gene Q1 Applied Microbiology and Biotechnology Article 03 medical and health sciences GERMLINE MD Multidisciplinary parasitic diseases CRISPR education SPECIFICITY Gene TOOLS Genetics education.field_of_study Biotechnology Molecular Medicine Science & Technology HOMING ENDONUCLEASE GENES NUCLEASES biology QH Gene drive biology.organism_classification 3. Good health DROSOPHILA 030104 developmental biology Biotechnology & Applied Microbiology Gene Drive Technology Life Sciences & Biomedicine |
| Zdroj: | Nature biotechnology |
| ISSN: | 1087-0156 |
| Popis: | Gene-drive systems that enable super-Mendelian inheritance of a transgene have the potential to modify insect populations over a timeframe of a few years [AU please provide a real estimate, this seems vague]. We describe CRISPR-Cas9 endonuclease constructs that function as gene-drive systems in Anopheles gambiae, the main vector for malaria [AU:OK?]. We identified three genes (AGAP005958, AGAP011377 and AGAP007280) that confer a recessive female sterility phenotype upon disruption, and inserted into each locus CRISPR-Cas9 gene-drive constructs designed to target and edit each gene [AU:OK?]. For each locus targeted we observed strong gene drive at the molecular level, with transmission rates to progeny of 91 to 99.6%. Population modelling and cage experiments indicate that a CRISPR-Cas9 construct targeting one of these loci, AGAP007280, meets the minimum requirement for a gene drive targeting female reproduction in an insect population. These findings could expedite the development of gene drives to control suppress mosquito populations to levels that do not support malaria transmission. |
| Databáze: | OpenAIRE |
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