A bovine protocol for training professionals in preimplantation genetic diagnosis using polymerase chain reaction
| Autor: | L. Kulay, Andréa Cristina de Moraes, Maria Regina Torloni, Carlos Gilberto Almodin, Antonio Fernandes Moron, Vânia C. Minguetti-Câmara |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_treatment
Sexing Biology Preimplantation genetic diagnosis Polymerase Chain Reaction law.invention Andrology Human reproduction Human fertilization Education Professional law Medical Laboratory Personnel medicine Animals Humans Preimplantation Diagnosis Polymerase chain reaction In vitro fertilisation Teaching Obstetrics and Gynecology Embryo Blastomere Sex Determination Processes Embryo Mammalian Molecular biology Reproductive Medicine Cattle Female |
| Zdroj: | Fertility and Sterility. 84:895-899 |
| ISSN: | 0015-0282 |
| Popis: | Objective To develop a bovine protocol for training in preimplantation genetic diagnosis (PGD) using PCR. Design Randomized study. Setting Human reproduction PCR laboratory. Patient(s) Cow ovaries obtained from slaughterhouses. Intervention(s) The ovaries were punctured and the oocytes were matured and submitted to in vitro fertilization. On the third day after fertilization, the embryos were biopsied and 1–2 blastomeres removed. A blastomere and the rest of the embryo were submitted to PCR for sex determination. Main Outcome Measure(s) Establishment of a possible training protocol. Result(s) A total of 50 embryos and 50 biopsied blastomeres were submitted to DNA amplification for sexing. Of the 50 embryos, 41 (82%) achieved successful DNA amplification and 9 (18%) did not. Of the 50 biopsies, 31 (62%) amplified and 19 (38%) did not. In 27 (65.9%) of the 41 embryos with DNA amplification, sex was identified as female and in 14 (34.1%) as male. In 40 cases (80%) amplification and sex determination were successful in both embryos and blastomeres. Sex was identical in all these cases. Conclusion(s) This training model seems to be useful in identifying mistakes and difficulties and improving the professional's performance in the various stages of preimplantation genetic diagnosis. |
| Databáze: | OpenAIRE |
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