Role of extracellular vesicles from adipose tissue‐ and bone marrow‐mesenchymal stromal cells in endothelial proliferation and chondrogenesis

Autor: Cansu Gorgun, Daniele Reverberi, Roberta Tasso, Maria Elisabetta Federica Palamà, Katia Cortese, Maria Cristina Gagliani, Chiara Gentili
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Medicine (General)
Adipose Stem Cells/VSF
Adipose tissue
regenerative medicine
Regenerative medicine
03 medical and health sciences
0302 clinical medicine
R5-920
Bone Marrow
Tissue‐specific Progenitor and Stem Cells
medicine
Bone Marrow Stem Cells
Endochondral ossification
Cells
Cultured
030304 developmental biology
Cell Proliferation
Bone growth
0303 health sciences
cartilage and bone development
QH573-671
Chemistry
Mesenchymal stem cell
Mesenchymal Stem Cells
ex vivo metatarsal culture model
extracellular vesicles
mesenchymal stromal cells
Cell Biology
General Medicine
Chondrogenesis
3. Good health
Cell biology
medicine.anatomical_structure
Adipose Tissue
030220 oncology & carcinogenesis
cartilage and bone development
ex vivo metatarsal culture model
extracellular vesicles
mesenchymal stromal cells
regenerative medicine
Bone marrow
Cytology
Ex vivo
Developmental Biology
Zdroj: Stem Cells Translational Medicine, Vol 10, Iss 12, Pp 1680-1695 (2021)
Stem Cells Translational Medicine
STEM CELLS Transl Med
ISSN: 2157-6564
2157-6580
Popis: The secretome of mesenchymal stromal cells (MSCs) derived from different tissue sources is considered an innovative therapeutic tool for regenerative medicine. Although adipose tissue‐and bone marrow‐derived MSCs (ADSCs and BMSCs, respectively) share many biological features, the different tissue origins can be mirrored by variations in their secretory profile, and in particular in the secreted extracellular vesicles (EVs). In this study, we carried out a detailed and comparative characterization of middle‐ and small‐sized EVs (mEVs and sEVs, respectively) released by either ADSCs or BMSCs. Their involvement in an endochondral ossification setting was investigated using ex vivo metatarsal culture models that allowed to explore both blood vessel sprouting and bone growth plate dynamics. Although EVs separated from both cell sources presented similar characteristics in terms of size, concentration, and marker expression, they exhibited different characteristics in terms of protein content and functional effects. ADSC‐EVs overexpressed pro‐angiogenic factors in comparison to the BMSC‐counterpart, and, consequently, they were able to induce a significant increase in endothelial cord outgrowth. On the other hand, BMSC‐EVs contained a higher amount of pro‐differentiation and chemotactic proteins, and they were able to prompt growth plate organization. The present study highlights the importance of selecting the appropriate cell source of EVs for targeted therapeutic applications.
This study shows how the differences among bone marrow‐ and adipose tissue‐derived mesenchymal stromal cells (BMSCs and ADSCs, respectively) are mirrored in the corresponding extracellular vesicles (EVs). The role of ADSCs‐ and BMSCs‐derived EVs in an endochondral ossification setting was investigated using ex vivo metatarsal culture models that allowed to explore both blood vessel sprouting and bone growth plate dynamics.
Databáze: OpenAIRE
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