The Quantitative Assessment of the Secreted IgG Repertoire after Recall to Evaluate the Quality of Immunizations
| Autor: | Klaus Eyer, Jérôme Bibette, Carlos Castrillon, Guilhem Chenon, Pierre Bruhns, Andrew D. Griffiths, Jean Baudry |
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| Přispěvatelé: | Institute of Pharmaceutical Sciences [ETH Zürich] (IPW), Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich), Laboratoire Colloïdes et Matériaux Divisés (LCMD), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Chimie-Biologie-Innovation (UMR 8231) (CBI), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Anticorps en thérapie et pathologie - Antibodies in Therapy and Pathology, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Plymouth University, This work was supported by funding from the The Branco Weiss Fellowship – Society in Science and funding from the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Program (Grant 80336) to K.E. This work has also received support from the Institut Pierre-Gilles de Gennes through Laboratoire d’Excellence, Investissements d’Avenir Programs ANR-10-IDEX-0001-02 PSL, ANR-10-EQPX-34, and ANR-10-LABX-31, and the ERC–Seventh Framework Program (ERC-2013-CoG 616050 to P.B.). C.C. acknowledges financial support from Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica, Peru., ANR-10-IDEX-0001,PSL,Paris Sciences et Lettres(2010), ANR-10-EQPX-0034,IPGG,Institut Pierre Gilles de Gennes pour la microfluidique(2010), European Project: 616050,EC:FP7:ERC,ERC-2013-CoG,MYELOSHOCK(2014), Centre National de la Recherche Scientifique (CNRS)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
MESH: Spleen
Immunology MESH: Mice Inbred BALB C Spleen Biology 03 medical and health sciences Mice 0302 clinical medicine Phenotypic analysis Novel Immunological Methods medicine Quantitative assessment Immunology and Allergy Animals MESH: Animals MESH: Mice 030304 developmental biology MESH: Immunoglobulin G 0303 health sciences Mice Inbred BALB C Recall Repertoire 3. Good health Vaccination Titer medicine.anatomical_structure Immunization Evaluation Studies as Topic 030220 oncology & carcinogenesis Immunoglobulin G MESH: Evaluation Studies as Topic MESH: Immunization [SDV.IMM]Life Sciences [q-bio]/Immunology Female MESH: Female [PHYS.COND.CM-SCM]Physics [physics]/Condensed Matter [cond-mat]/Soft Condensed Matter [cond-mat.soft] |
| Zdroj: | Journal of Immunology Journal of Immunology, 2020, 205 (4), pp.1176-1184. ⟨10.4049/jimmunol.2000112⟩ The Journal of Immunology, 205 (4) The Journal of Immunology J Immunol Journal of Immunology, Publisher : Baltimore : Williams & Wilkins, c1950-. Latest Publisher : Bethesda, MD : American Association of Immunologists, 2020, 205 (4), pp.1176-1184. ⟨10.4049/jimmunol.2000112⟩ |
| ISSN: | 0022-1767 1550-6606 |
| DOI: | 10.4049/jimmunol.2000112⟩ |
| Popis: | One of the major goals of vaccination is to prepare the body to rapidly secrete specific Abs during an infection. Assessment of the vaccine quality is often difficult to perform, as simple measurements like Ab titer only partly correlate with protection. Similarly, these simple measurements are not always sensitive to changes in the preceding immunization scheme. Therefore, we introduce in this paper a new, to our knowledge, method to assay the quality of immunization schemes for mice: shortly after a recall with pure Ag, we analyze the frequencies of IgG-secreting cells (IgG-SCs) in the spleen, as well as for each cells, the Ag affinity of the secreted Abs. We observed that after recall, appearance of the IgG-SCs within the spleen of immunized mice was fast ( |
| Databáze: | OpenAIRE |
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