Determination of plasma levels of two aromatic retinoic acid analogues with antipsoriatic activity by high-performance liquid chromatography
| Autor: | Göran Palmskog |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Detection limit
Chromatography Metabolite Uv absorption Retinoic acid Tretinoin General Chemistry Plasma levels Reference Standards High-performance liquid chromatography Acitretin chemistry.chemical_compound Kinetics chemistry Etretinate Humans Spectrophotometry Ultraviolet Diethyl ether Quantitative analysis (chemistry) Chromatography High Pressure Liquid |
| Zdroj: | Journal of chromatography. 221(2) |
| Popis: | A method is described for the quantitative analysis in plasma of Ro 10-9359, an aromatic retinoic acid analogue, ethyl all-trans-9-(4-methoxy-2,3,6-trimethylphenyl)-3,7-dimethyl-2,4,6,8-nonatetraenoate and its major metabolite Ro 10-1670, all-trans-9-(4-methoxy-2,3,6-trimethylphenyl)-3,7-dimethyl-2,3,6,8-nonatetraenoic acid. The compounds are extracted from patient plasma with diethyl ether, separated on a reversed-phase column and detected and quantified by their UV absorption. The experimental error is below 9% in the concentration range 42-445 ng/ml. The detection limit is about 10 ng/ml. The method was applied to the analysis of plasma levels in healthy volunteers, receiving 75 mg orally. |
| Databáze: | OpenAIRE |
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