Inhibitory effects of Porphyra dentata extract on 3T3-L1 adipocyte differentiation
| Autor: | Da hye Jang, Su Yeon Lee, Su-Young Choi, Suk Jun Lee, Sung Hak Kim, Jeong-Yong Cho |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
anti-obesity
Veterinary (miscellaneous) Cellular differentiation Biochemistry Genetics and Molecular Biology (miscellaneous) adipogenesis chemistry.chemical_compound 3t3-l1 Lipid droplet Enhancer binding Oil Red O Viability assay adipocyte protein 2 adipocyte differentiation lcsh:SF1-1100 Ecology biology Chemistry 3T3-L1 Molecular biology Adipogenesis porphyra dentata biology.protein Animal Science and Zoology lcsh:Animal culture Food Science Research Article |
| Zdroj: | Journal of Animal Science and Technology, Vol 62, Iss 6, Pp 854-863 (2020) Journal of Animal Science and Technology |
| ISSN: | 2055-0391 2672-0191 |
| Popis: | This study was aimed to investigate the inhibitory effects of Porphyra dentata (P. dentata) extract on the adipogenesis of 3T3-L1 cells and evaluate its anti-obesity effect. The proliferation of 3T3-L1 cells and differentiation of adipocytes under treatment of P. dentata extract was examined by measuring the cell viability using alamarBlue assay and lipid droplets by Oil Red O staining. Results showed that P. dentata extract has no cytotoxicity effect and lipid droplets formation decreased in a concentration-dependent manner in 3T3-L1 cells. It has been confirmed that transcription factors affecting lipid accumulation and anti-adipogenic effects during cell differentiation are linked to P. dentata extract. We observed that P. dentata shows lowering the mRNA expression of peroxisome proliferator-activated receptor γ2 (PPARγ2), CCAAT/enhancer binding protein α (C/EBPα) that adipogenesis-associated key transcription factors and inhibiting adipogenesis in the early stages of differentiation. Treating the cells with P. dentata did not only suppressed PPARγ2 and C/EBPα but also significantly decreased the mRNA expression of adiponectin, Leptin, fatty acid synthase, adipocyte protein 2, and Acetyl-coA carboxylase 1. Overall, the P. dentata extract demonstrated inhibitory property in adipogenesis, which has a potential effect in anti-obesity in 3T3-L1 cells. |
| Databáze: | OpenAIRE |
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