Effect of Chronic Dietary Ethanol Consumption on Colonic Cancer in Rats Induced by 1,1-Dimethylhydrazine
| Autor: | Mutsumi Tsuchishima, Masayuki Fukura, Nobuhiko Hayashi, Shujiro Takase, Hirokazu Yano, Mikihiro Tsutsumi |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
endocrine system
medicine.medical_specialty Pathology Colon Colorectal cancer Medicine (miscellaneous) Toxicology chemistry.chemical_compound Internal medicine medicine Dimethylhydrazine Animals Intestinal Mucosa Rats Wistar Carcinogen Dimethylhydrazines Cocarcinogenesis Ethanol business.industry Cytochrome P-450 CYP2E1 Histology CYP2E1 medicine.disease Rats Staining Alcoholism Psychiatry and Mental health Cell Transformation Neoplastic Endocrinology Liver chemistry Colonic Neoplasms Carcinogens business Aberrant crypt foci |
| Zdroj: | Alcoholism: Clinical and Experimental Research. 31:S72-S76 |
| ISSN: | 1530-0277 0145-6008 |
| DOI: | 10.1111/j.1530-0277.2006.00290.x |
| Popis: | Background: The pathogenetic correlation between chronic alcohol consumption and development of colon cancer is not clear. The role of alcohol abuse in the carcinogenic action of 1,1-dimethylhydrazine (DMH), which induces tumors in the colon, was evaluated. Methods: Twenty male rats were fed liquid diets containing ethanol or carbohydrates for 39 weeks. DMH (20 mg/kg body weight, once a week) was injected subcutaneously from the 5th to the 20th week. Pair feeding was stopped at 10:00 am and DMH was administered at 02:00 pm. Ethanol was not detected in the blood at the time of injection. Liquid diets were provided again at 05:00 pm until 10:00 am next day. The animals were killed at the end of the 39th week, and the colons were removed for examination for the number of aberrant crypt foci (ACF) by methylene blue staining. Tissue sections were stained for histology and cytochrome P4502E1 (CYP2E1) expression. Results: The number of ACF in colons obtained from ethanol-fed rats with DMH was 24 (n=5, 4.4±2.5/rat), which was significantly (p |
| Databáze: | OpenAIRE |
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