Bone marrow vs Wharton’s jelly mesenchymal stem cells in experimental sepsis: a comparative study
| Autor: | Corentine Alauzet, Danièle Bensoussan, Clémence Yguel, Loïc Reppel, Jean-Louis Merlin, Lucie Jolly, Sébastien Gibot, Amir Boufenzer, Caroline Laroye, Lisiane Cunat |
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| Přispěvatelé: | Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Unité de Thérapie Cellulaire et Tissulaire [CHU Nancy], Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Défaillance Cardiovasculaire Aiguë et Chronique (DCAC), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL), INOTREM, Stress, Immunité, Pathogènes (SIMPA), Université de Lorraine (UL), Institut de Cancérologie de Lorraine - Alexis Vautrin [Nancy] (UNICANCER/ICL), UNICANCER, Département d’Anatomie et Cytologie Pathologiques [CHRU Nancy], Service de Réanimation Médicale [CHRU Nancy] |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Pathology Medicine (miscellaneous) Mice 0302 clinical medicine Wharton's jelly Leukocytes Medicine lcsh:QD415-436 Wharton Jelly Cecum Cells Cultured lcsh:R5-920 Wharton’s jelly Tissue source 3. Good health medicine.anatomical_structure 030220 oncology & carcinogenesis Molecular Medicine medicine.symptom Stem cell lcsh:Medicine (General) medicine.medical_specialty Peritonitis Bone Marrow Cells Punctures [SDV.BC]Life Sciences [q-bio]/Cellular Biology Mesenchymal Stem Cell Transplantation Biochemistry Genetics and Molecular Biology (miscellaneous) Sepsis lcsh:Biochemistry 03 medical and health sciences Animals Humans Bone marrow Ligation Inflammation business.industry Septic shock Research Mesenchymal stem cell Organ dysfunction Cell Biology medicine.disease Mice Inbred C57BL 030104 developmental biology Mesenchymal stem cells business |
| Zdroj: | Current Stem Cell Research & Therapy Current Stem Cell Research & Therapy, Bentham Science Publishers Ltd, 2019, 10 (1), pp.192. ⟨10.1186/s13287-019-1295-9⟩ Stem Cell Research & Therapy, Vol 10, Iss 1, Pp 1-11 (2019) Stem Cell Research & Therapy |
| ISSN: | 1574-888X |
| DOI: | 10.1186/s13287-019-1295-9⟩ |
| Popis: | International audience; BACKGROUND: The use of mesenchymal stem cells (MSCs) is being extensively studied in clinical trials in the setting of various diseases including diabetes, stroke, and progressive multiple sclerosis. The unique immunomodulatory properties of MSCs also point them as a possible therapeutic tool during sepsis and septic shock, a devastating syndrome associated with 30-35% mortality. However, MSCs are not equal regarding their activity, depending on their tissue origin. Here, we aimed at comparing the in vivo properties of MSCs according to their tissue source (bone marrow (BM) versus Wharton's jelly (WJ)) in a murine cecal ligation and puncture (CLP) model of sepsis that mimics a human peritonitis. We hypothesized that MSC properties may vary depending on their tissue source in the setting of sepsis.METHODS: CLP, adult, male, C57BL/6 mice were randomized in 3 groups receiving respectively 0.25 × 106 BM-MSCs, 0.25 × 106 WJ-MSCs, or 150 μL phosphate-buffered saline (PBS) intravenously 24 h after the CLP procedure.RESULTS: We observed that both types of MSCs regulated leukocyte trafficking and reduced organ dysfunction, while only WJ-MSCs were able to improve bacterial clearance and survival.CONCLUSION: This study highlights the importance to determine the most appropriate source of MSCs for a given therapeutic indication and suggests a better profile for WJ-MSCs during sepsis. |
| Databáze: | OpenAIRE |
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