tRNA methylation A rapid and simple method for determination of total radioactivity and methylated base distribution in the same sample
| Autor: | Ghislaine Pham-Coeur-Joly, Francois Nau |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
chemistry.chemical_classification
Radioisotope Dilution Technique S-Adenosylmethionine tRNA Methyltransferases TRNA methylation Chromatography Methyltransferase Base (chemistry) Sample (material) Dimethyl-POPOP Methylation Biochemistry Genetics and Molecular Biology (miscellaneous) RNA Transfer Biochemistry chemistry Transfer RNA Escherichia coli Sample preparation Carbon Radioisotopes Chromatography Thin Layer |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis. 653:299-302 |
| ISSN: | 0005-2787 |
| DOI: | 10.1016/0005-2787(81)90166-0 |
| Popis: | A simple and rapid method is described for the analysis of in vitro methylated tRNA. The total radioactivity is first determined after trichloroacetic acid-precipitation and filtration. Then the tRNA is hydrolyzed while still on the filter, and the bases are separated by thin-layer chromatography. This method eliminates the need for duplicate sample preparation, and has proved especially useful when the amount of tRNA and/or tRNA methylase is limited. |
| Databáze: | OpenAIRE |
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