GP.MOT: A novel glycophorin variant identified in a Japanese blood donor
| Autor: | Akira Oda, Takafumi Kimura, Kazumi Isa, Nelson Hirokazu Tsuno, Yumi Suzuki, Ryuichi Yabe, Kenichi Ogasawara, Makoto Uchikawa |
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| Rok vydání: | 2021 |
| Předmět: |
Proband
Immunology Blood Donors symbols.namesake Antigen Japan Immunology and Allergy Glycophorin Humans Amino Acid Sequence Glycophorins Allele Peptide sequence Alleles Sanger sequencing GYPA GYPB biology Base Sequence Chemistry Genetic Variation Hematology Sequence Analysis DNA Molecular biology symbols biology.protein MNSs Blood-Group System |
| Zdroj: | TransfusionREFERENCES. 61(10) |
| ISSN: | 1537-2995 |
| Popis: | BACKGROUND In this study, we identified a novel glycophorin variant (GP.MOT) in a Mia -positive Japanese blood donor. The proband with this glycophorin variant was discovered by antigen screening of samples from 475,493 Japanese blood donors using monoclonal anti-Mia . STUDY DESIGN AND METHODS Standard serological techniques and flow cytometry were performed. GP.MOT RBCs were examined by immunoblotting using anti-GPA, anti-MUT or anti-Mur. Genome DNA was extracted from whole blood, and the GYPA/GYPB was analyzed by polymerase chain reactions and Sanger sequencing. RESULTS The MNS blood group of the proband was M + N + w S-s + with the presence of other low-frequency antigens including Mia , Mur, MUT, and KIPP. A 43-kDa molecule, which is almost equivalent in size to glycophorin A (GPA), was identified by immunoblotting using monoclonal anti-MUT and anti-Mur. Sanger sequencing clearly indicated that the proband had two different GYPA*M alleles at SNP rs62334651 (GYPA*M232 + 55A and GYPA*M232 + 55G), as well as a GYP(B-A) hybrid allele (GYP*MOT) with breakpoints located on pseudoexon 3 of GYPB from c.210 to c.219. DISCUSSION We identified a hybrid glycophorin GP.MOT with the deduced unique amino acid sequence GPB (20-45)-GPΨB (46-70)-GPA (71-149), which has not been previously reported. |
| Databáze: | OpenAIRE |
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