Apoptosis and Myofibroblast Expression in Human Glomerular Disease: A Possible Link with Transforming Growth Factor-Beta-1
| Autor: | Graham L. Thomas, Athanassios C. Tsamandas, Sotiris Tsakas, Dimitrios S. Goumenos, John G. Vlachojannis, Dionysis S Bonikos, Florentia Sotsiou, A. Meguid El Nahas |
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| Rok vydání: | 2002 |
| Předmět: |
Adult
Male Programmed cell death Glomerulonephritis Membranoproliferative Apoptosis Biology Transforming Growth Factor beta1 Pathogenesis Glomerulonephritis Transforming Growth Factor beta medicine Renal fibrosis Humans Aged Glomerulosclerosis Focal Segmental Muscle Smooth Transforming growth factor beta Fibroblasts Middle Aged medicine.disease Fibrosis Immunohistochemistry Actins Immunology Cancer research biology.protein Female Myofibroblast Kidney disease |
| Zdroj: | Nephron. 92:287-296 |
| ISSN: | 2235-3186 1660-8151 |
| Popis: | Background/Aims: The pathophysiological pathways involved in the pathogenesis and evolution of renal fibrosis, have not been fully elucidated. Transforming growth factor-beta1 (TGF-β1) is involved in the development of renal scarring. Apoptosis is responsible for intrinsic cell deletion observed in end-stage kidney disease. Myofibroblasts are involved in the development of renal fibrosis. This study investigates whether there is a potential relationship between apoptosis, myofibroblast infiltration and TGF-β1 expression in the kidney of patients with glomerulonephritis (GN). Methods: Forty patients with various types of GN were included in the study. Myofibroblasts and TGF-β1 positive cells were detected in kidney biopsies by immunohistochemistry, while apoptotic cells were detected by the in situ end labelling of fragmented DNA. Results: Myofibroblasts were identified in the glomeruli of some patients with severe mesangioproliferative GN and glomerulosclerosis but a more intensive myofibroblast expression was found in the renal interstitium. TGF-β1 was expressed in the cytoplasm of tubular epithelial cells, in the renal interstitium and in the glomeruli of patients with GN. Apoptotic cells were mainly detected in the tubules and interstitium and were present in areas with intense myofibroblast infiltration. Positive correlations were observed between the intensity of myofibroblast expression in the interstitium and apoptosis in the tubulointerstitial area (r = 0.521, p < 0.01) as well as TGF-β1 expression (r = 0.462, p < 0.05) and degree of renal impairment (r = 0.430, p < 0.05). Conclusions: These observations suggest that myofibroblast infiltration and apoptosis along with TGF-β1 expression are associated with the development of interstitial fibrosis in patients with glomerular disease. |
| Databáze: | OpenAIRE |
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