Natural trypanocidal product produced by endophytic fungi through co-culturing
| Autor: | Ivo Santana Caldas, Jaine H.H. Luiz, Felipe Moura Silva, Elda Gonçalves dos Santos, Marcela Kurina-Sanz, Eliane de Oliveira Silva, Cynthia Magallanes-Noguera, Jainara Santos do Nascimento |
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| Rok vydání: | 2019 |
| Předmět: |
Handroanthus impetiginosus
Silent gene NATURAL PRODUCT Trypanosoma cruzi CO-CULTURE FUNGAL Phanerochaete Tabebuia Microbiology Plant use of endophytic fungi in defense purl.org/becyt/ford/1 [https] Botany purl.org/becyt/ford/1.4 [https] Endophytes Axenic Talaromyces purpurogenus Co culturing biology Terpenes General Medicine biology.organism_classification Isolation (microbiology) Trypanocidal Agents Coculture Techniques Plant Leaves Talaromyces ENDOPHYTE |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 1874-9356 |
| Popis: | Endophytic fungi live inside vegetal tissues without causing damage to the host plant and may provide lead compounds for drug discovery. The co-culture of two or more endophytic fungi can trigger silent gene clusters, which could lead to the isolation of bioactive compounds. In this study, two endophytic strains isolated from Handroanthus impetiginosus leaves, identified as Talaromyces purpurogenus H4 and Phanerochaete sp. H2, were grown in mixed and axenic cultures. The meroterpenoid austin was detected only in the extracts from the mixed culture. Once isolated, austin displayed very interesting trypanocidal activity, with an IC50 value of 36.6 ± 1.2 μg/mL against Trypanosoma cruzi in the epimastigote form. The results obtained highlight the importance of the co-culturing of endophytic fungi to obtain natural bioactive products. The findings also enhance our understanding of the ecological relationships between endophytic fungi. Fil: do Nascimento, Jainara Santos. Universidade Federal da Bahia; Brasil Fil: Silva, Felipe Moura. Universidade Federal da Bahia; Brasil Fil: Magallanes Noguera, Cynthia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina Fil: Kurina Sanz, Marcela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina Fil: dos Santos, Elda Gonçalves. Universidade Federal da Bahia; Brasil Fil: Caldas, Ivo Santana. Universidade Federal da Bahia; Brasil Fil: Luiz, Jaine Honorata Hortolan. Universidade Federal da Bahia; Brasil Fil: Silva, Eliane de Oliveira. Universidade Federal da Bahia; Brasil |
| Databáze: | OpenAIRE |
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