Exposure to 50 Hz Extremely-Low-Frequency Magnetic Fields Induces No DNA Damage in Cells by Gamma H2AX Technology
| Autor: | Qun-li Zeng, Bing Zhu, Hong Xu, Yunyun Shen, Weiyan Liu, Shanshan Xu, Ye Lv, Shuchang Chen |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Article Subject DNA damage Cell Immunofluorescence General Biochemistry Genetics and Molecular Biology Umbilical vein Histones 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Western blot Molecular marker Human Umbilical Vein Endothelial Cells medicine Humans Cells Cultured General Immunology and Microbiology Amnion medicine.diagnostic_test Chemistry Epithelial Cells General Medicine Fibroblasts respiratory system Molecular biology In vitro Magnetic Fields 030104 developmental biology medicine.anatomical_structure Microscopy Fluorescence 030220 oncology & carcinogenesis Medicine DNA Damage Research Article |
| Zdroj: | BioMed Research International, Vol 2021 (2021) BioMed Research International |
| ISSN: | 2314-6133 |
| DOI: | 10.1155/2021/8510315 |
| Popis: | The current results for extremely-low-frequency magnetic fields (ELF-MF) on DNA damage are still debated. A sensitive indicator and systematic research are needed to assess the effects of ELF-MF. In this study, we used γH2AX as an early and sensitive molecular marker to evaluate the DNA damage effects of ELF-MF in vitro. Human amnion epithelial cells (FLs), human skin fibroblast cells (HSFs), and human umbilical vein endothelial cells (HUVECs) were exposed to 50 Hz ELF-MF at 0.4, 1, and 2 mT for 15 min, 1 h, and 24 h, respectively. After exposure, cells were subjected to γH2AX immunofluorescence and western blot. The results showed no significant difference in the average number of foci per cell, the percentage of γH2AX foci-positive cells, or the expression of γH2AX between the sham and 50 Hz ELF-MF exposure groups ( P > 0.05 ). In conclusion, 50 Hz ELF-MF did not induce DNA damage in FLs, HSFs, or HUVECs, which was independent of the intensity or duration of the exposure. |
| Databáze: | OpenAIRE |
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