Salicylic acid production in response to biotic and abiotic stress depends on isochorismate in Nicotiana benthamiana
| Autor: | Jérémy Catinot, Jean-Pierre Métraux, Eliane Abou-Mansour, Antony Buchala |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Nicotiana
0106 biological sciences UV stress Chorismic Acid Isochorismate synthase Molecular Sequence Data Biophysics Nicotiana benthamiana Polymerase Chain Reaction Pathogen stress 01 natural sciences Biochemistry 03 medical and health sciences chemistry.chemical_compound Structural Biology Phylloquinone Cyclohexenes Tobacco Genetics Gene silencing Arabidopsis thaliana Amino Acid Sequence Intramolecular Transferases Molecular Biology DNA Primers 030304 developmental biology 0303 health sciences Base Sequence Sequence Homology Amino Acid biology Abiotic stress fungi food and beverages Salicylic acid Cell Biology Biotic stress biology.organism_classification chemistry biology.protein 010606 plant biology & botany |
| Popis: | Salicylic acid (SA) is an important signal involved in the activation of defence responses against abiotic and biotic stress. In tobacco, benzoic acid or glucosyl benzoate were proposed to be precursors of SA. This is in sharp contrast with studies in Arabidopsis thaliana, where SA derives from isochorismate. We have determined the importance of isochorismate for SA biosynthesis in Nicotiana benthamiana using virus-induced gene silencing of the isochorismate synthase (ICS) gene. Plants with silenced ICS expression do not accumulate SA after exposure to UV or to pathogen stress. Plants with silenced ICS expression also exhibit strongly decreased levels of phylloquinone, a product of isochorismate. Our data provide evidence for an isochorismate-derived synthesis of SA in N. benthamiana |
| Databáze: | OpenAIRE |
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