Linking genotype to phenotype on beads: high throughput selection of peptides with biological function
| Autor: | Graham S. Ogg, Hongbing Yang, Guillaume Stewart-Jones, Lucy Dorrell, Lai Kin Derek Wan, Li-Chieh Huang, Xiaoyan Pan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Receptors
CCR5 Anti-HIV Agents Microbial Sensitivity Tests Plasma protein binding Computational biology HIV Envelope Protein gp120 Biology Article chemistry.chemical_compound Receptors HIV Peptide Library In vivo High-Throughput Screening Assays Humans Avidity Peptide library chemistry.chemical_classification Multidisciplinary Biomolecule Reproducibility of Results Molecular biology Microspheres In vitro Kinetics chemistry HIV-1 Peptides beta 2-Microglobulin DNA Protein Binding |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Although peptides are well recognised biological molecules in vivo, their selection from libraries is challenging because of relative low affinity whilst in linear conformation. We hypothesized that multiplexed peptides and DNA on the surface of beads would provide a platform for enhanced avidity and the selection of relevant peptides from a library (ORBIT bead display). Using human immunodeficiency virus (HIV-1) gp120 as a target, we identify peptides that inhibit HIV-1 replication in vitro through blocking of protein:protein interaction with the co-receptor CCR5. The bead display approach has many potential applications for probing biological systems and for drug lead development. |
| Databáze: | OpenAIRE |
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