Coupling Two Different Nucleic Acid Circuits in an Enzyme-Free Amplifier
| Autor: | Bingling Li, Yu Sherry Jiang, Andrew D. Ellington, Xi Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Inverted Repeat Sequences
Immobilized Nucleic Acids Biotin Pharmaceutical Science 02 engineering and technology 010402 general chemistry G-quadruplex 01 natural sciences Article Analytical Chemistry lcsh:QD241-441 chemistry.chemical_compound lcsh:Organic chemistry Drug Discovery hybridization chain reaction (HCR) 96-well plate Physical and Theoretical Chemistry Fluorescent Dyes Peroxidase Electronic circuit catalyzed hairpin assembly (CHA) enzyme-free Amplifier Organic Chemistry Nucleic acid sequence DNA Catalytic Nucleic acid amplification technique 021001 nanoscience & nanotechnology DNA Concatenated Combinatorial chemistry 0104 chemical sciences G-Quadruplexes Immobilized Proteins chemistry Biochemistry Chemistry (miscellaneous) Nucleic acid Hemin Molecular Medicine Streptavidin 0210 nano-technology Nucleic Acid Amplification Techniques DNA |
| Zdroj: | Molecules, Vol 17, Iss 11, Pp 13211-13220 (2012) Molecules Molecules; Volume 17; Issue 11; Pages: 13211-13220 |
| ISSN: | 1420-3049 |
| Popis: | DNA circuits have proven to be useful amplifiers for diagnostic applications, in part because of their modularity and programmability. In order to determine whether different circuits could be modularly stacked, we used a catalytic hairpin assembly (CHA) circuit to initiate a hybridization chain reaction (HCR) circuit. In response to an input nucleic acid sequence, the CHA reaction accumulates immobilized duplexes and HCR elongates these duplexes. With fluorescein as a reporter each of these processes yielded 10-fold signal amplification in a convenient 96-well format. The modular circuit connections also allowed the output reporter to be readily modified to a G-quadruplex-DNAzyme that yielded a fluorescent signal. |
| Databáze: | OpenAIRE |
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