Cardiac muscle tissue engineering: toward an in vitro model for electrophysiological studies
| Autor: | Maria Papadaki, Lisa E. Freed, Nenad Bursac, Solomon R. Eisenberg, R. Carrier, Richard J. Cohen, Frederick J. Schoen, Gordana Vunjak-Novakovic |
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| Rok vydání: | 1999 |
| Předmět: |
Aging
Pathology medicine.medical_specialty Polymers Physiology Heart Ventricles Biomedical Engineering Biology In vitro model Rats Sprague-Dawley Culture Techniques Physiology (medical) medicine Animals Myocyte Neonatal rat Histological Techniques Cardiac muscle Anatomy Papillary Muscles In vitro Rats Electrophysiology Biodegradation Environmental medicine.anatomical_structure Animals Newborn Circulatory system Cardiac muscle tissue Cardiology and Cardiovascular Medicine |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 277:H433-H444 |
| ISSN: | 1522-1539 0363-6135 |
| DOI: | 10.1152/ajpheart.1999.277.2.h433 |
| Popis: | The objective of this study was to establish a three-dimensional (3-D) in vitro model system of cardiac muscle for electrophysiological studies. Primary neonatal rat ventricular cells containing lower or higher fractions of cardiac myocytes were cultured on polymeric scaffolds in bioreactors to form regular or enriched cardiac muscle constructs, respectively. After 1 wk, all constructs contained a peripheral tissue-like region (50–70 μm thick) in which differentiated cardiac myocytes were organized in multiple layers in a 3-D configuration. Indexes of cell size (protein/DNA) and metabolic activity (tetrazolium conversion/DNA) were similar for constructs and neonatal rat ventricles. Electrophysiological studies conducted using a linear array of extracellular electrodes showed that the peripheral region of constructs exhibited relatively homogeneous electrical properties and sustained macroscopically continuous impulse propagation on a centimeter-size scale. Electrophysiological properties of enriched constructs were superior to those of regular constructs but inferior to those of native ventricles. These results demonstrate that 3-D cardiac muscle constructs can be engineered with cardiac-specific structural and electrophysiological properties and used for in vitro impulse propagation studies. |
| Databáze: | OpenAIRE |
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