Identification of metabolite biomarkers in serum of rats exposed to chlorpyrifos and cadmium
Autor: | Pan Wang, Yi-Jun Wu, Lin Yang, Ming-Yuan Xu, Ying-Jian Sun |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Male Cholinesterase Reactivators Time Factors Metabolite chemistry.chemical_element lcsh:Medicine 010501 environmental sciences Pharmacology 01 natural sciences Biochemistry Gas Chromatography-Mass Spectrometry Article Serine Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Metabolomics Animals Toxicity Tests Chronic lcsh:Science 0105 earth and related environmental sciences Cadmium Multidisciplinary lcsh:R Environmental Exposure Malonates 030104 developmental biology chemistry Chlorpyrifos Toxicity Biomarker (medicine) Environmental Pollutants lcsh:Q Gas chromatography–mass spectrometry Propionates Energy Metabolism Systems biology Biomarkers |
Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-10 (2020) Scientific Reports |
ISSN: | 2045-2322 |
Popis: | Chlorpyrifos (CPF) and cadmium (Cd) are widespread environmental pollutants, which are often present in drinking water and foods. However, the combined effects of CPF and Cd were not entirely clear at present. There was also no biomarker available to diagnose the poisoning of the two chemicals at low dose for long-term exposures. In this study, we investigated the change of serum metabolites of rats with subchronic exposure to CPF, Cd, and CPF plus Cd using gas chromatography-mass spectrometer-based metabolomics approach. We performed a stepwise optimization algorithm based on receiver operating characteristic to identify serum metabolite biomarkers for toxic diagnosis of the chemicals at different doses after 90-day exposure. We found that aminomalonic acid was the biomarker for the toxicity of Cd alone administration, and serine and propanoic acid were unique biomarkers for the toxicities of CPF plus Cd administrations. Our results suggest that subchronic exposure to CPF and Cd alone, or in combination at their low doses, could cause disturbance of energy and amino acid metabolism. Overall, we have shown that analysis of serum metabolomics can make exceptional contributions to the understanding of the toxic effects following long-term low-dose exposure of the organophosphorus pesticide and heavy metal. |
Databáze: | OpenAIRE |
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