Changes in cell membrane properties and phospholipid fatty acids of bacillus subtilis induced by polyphenolic extract of Sanguisorba officinalis L
| Autor: | Gang Chen, Rui‐qi Wang, Su-ping Jian, Chen Sunni, Hui Xue, Hong-lin Zhu, Long Chen |
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| Rok vydání: | 2020 |
| Předmět: |
030309 nutrition & dietetics
Phospholipid Microbial Sensitivity Tests Bacillus subtilis Cell morphology Sanguisorba Cell membrane Cell wall 03 medical and health sciences chemistry.chemical_compound Minimum inhibitory concentration 0404 agricultural biotechnology Cell Wall Sanguisorba officinalis medicine Phospholipids 0303 health sciences biology Plant Extracts Chemistry Cell Membrane Fatty Acids Polyphenols 04 agricultural and veterinary sciences biology.organism_classification 040401 food science Anti-Bacterial Agents medicine.anatomical_structure Biochemistry Food Preservatives Antibacterial activity Food Science |
| Zdroj: | Journal of Food Science. 85:2164-2170 |
| ISSN: | 1750-3841 0022-1147 |
| Popis: | Sanguisorba officinalis L. (family Rosaceae, subfamily Rosoideae) is a plant found throughout Southern Europe, Northern Africa, and Eastern Asia. This study demonstrated the antibacterial activity of a purified polyphenolic extract (PPE) from S. officinalis L. against Bacillus subtilis using growth inhibitory and apoptosis assays, and investigated the antibacterial mechanism responsible for changes in cell membrane properties. Fourier transform infrared spectroscopy suggested that PPE altered the cell wall and membrane properties of B. subtilis. Further determination of cell membrane integrity and permeability revealed that B. subtilis membrane integrity was more severely damaged by PPE at the minimum inhibitory concentration (MIC) than at the minimum bactericidal concentrati on (MBC). Instead, PPE at the MBC reduced cell membrane fluidity by significantly decreasing the proportion of anteiso- and iso-branched phospholipid fatty acids (PLFAs) from 64.17 ± 0.28% and 27.23 ± 0.03% in the control to 5.57 ± 1.06% and 6.00 ± 1.40%, respectively (P |
| Databáze: | OpenAIRE |
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