Analysis of restriction enzyme-induced chromosomal aberrations by fluorescence in situ hybridization
| Autor: | William F. Morgan, Barbara L. Yates, Elma Abella Columna, James W. Evans, Amato J. Giaccia |
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| Rok vydání: | 1993 |
| Předmět: |
Epidemiology
DNA repair Health Toxicology and Mutagenesis Ring chromosome Population Chromosomal translocation Biology Translocation Genetic Giemsa stain Cell Line medicine Humans Ring Chromosomes education Metaphase In Situ Hybridization Fluorescence Genetics (clinical) Chromosome Aberrations education.field_of_study medicine.diagnostic_test X-Rays DNA Restriction Enzymes Molecular biology Restriction enzyme Chromosomes Human Pair 2 Chromosome Deletion Chromosomes Human Pair 4 DNA Damage Fluorescence in situ hybridization |
| Zdroj: | Environmental and Molecular Mutagenesis. 22:26-33 |
| ISSN: | 1098-2280 0893-6692 |
| DOI: | 10.1002/em.2850220106 |
| Popis: | Fluorescence in situ hybridization and Giemsa staining of metaphase chromosomes were used to determine the relative frequencies of symmetric exchange aberrations (translocations) and asymmetric exchange aberrations (rings, dicentrics, and polycentrics) after exposure of human lymphoblastoid cells to restriction enzymes or X-rays. The yield of symmetric exchanges was determined with the use of chromosome-specific probes for human chromosomes 2 or 4, which were hybridized to metaphase chromosomes from cells exposed to the enzymes PvuII, SacI, or XbaI or 3 or 5 Gy of X-rays. The yield of asymmetric exchanges was determined in Giemsa-stained metaphase chromosomes from the same enzyme-treated or irradiated cell population. About 1.5- to 3-fold more symmetric than asymmetric exchanges were induced after restriction enzyme treatment. However, after X-ray treatment the yield of dicentrics relative to the yield of reciprocal translocations was close to the expected 1:1 ratio. |
| Databáze: | OpenAIRE |
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