Treatment of medullary thyroid carcinoma by combined expression of suicide and interleukin-2 genes
| Autor: | F. Treilhou-Lahille, M. N. Soler, F. Lekmine, Gérard Milhaud, S. Lausson, David Klatzmann |
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| Rok vydání: | 1999 |
| Předmět: |
Ganciclovir
Interleukin 2 Cancer Research Genetic enhancement T-Lymphocytes Immunology Herpesvirus 1 Human Biology Antiviral Agents Thymidine Kinase medicine Immunology and Allergy Animals Thyroid Neoplasms Tumor-infiltrating lymphocytes Rats Inbred Strains Genetic Therapy Suicide gene medicine.disease Rats Oncology Medullary carcinoma Thymidine kinase Carcinoma Medullary Cancer research Interleukin-2 CD8 medicine.drug |
| Zdroj: | Cancer immunology, immunotherapy : CII. 48(2-3) |
| ISSN: | 0340-7004 |
| Popis: | Inherited medullary thyroid carcinomas (MTC) are aggressive and resistant to conventional chemo- and radiotherapies. We evaluated a novel strategy for treatment of MTC, combining “suicide” and interleukin-2 (IL-2) gene therapies. Tumors were produced in Wag/Rij rats by orthotopic injection of the rMTC 6–23 cell line, and/or derivatives expressing the herpes simplex virus 1 thymidine kinase (HSV1-TK) gene (rMTC-TK). Ganciclovir, a nucleoside analog selectively transformed to a toxic metabolite by HSV1-TK, totally eradicated rMTC-TK tumors in 60% of the animals. 1:1 rMTC and rMTC-TK mixed tumors were also strongly inhibited by ganciclovir (P < 0.05), indicating the occurrence of an efficient “bystander” effect in vivo. Double labelling of rMTC cell membranes and apoptotic nuclei revealed that, as with the TK+ cells, some TK− cells died by apoptosis. A 1:1 mixture of rMTC and rMTC-TK cells was administered to produce established tumors and then rMTC cells, transfected to express the IL-2 gene (rMTC-IL2), were inoculated. Combined ganciclovir and IL-2 treatment improved the inhibition of tumor growth compared to that following ganciclovir alone (86% compared to 54%, P < 0.05). This treatment also significantly enhanced macrophage activation and tumor infiltration by CD8+ and CD4+ T lymphocytes. These results open an avenue for combining suicide and immunoregulatory gene therapies for MTC management in man. |
| Databáze: | OpenAIRE |
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