Association of SARS-CoV-2 clades with clinical, inflammatory and virologic outcomes: An observational study
| Autor: | Lin-Fa Wang, Adrian Kang Eng Zheng, Siew-Wai Fong, Tze Minn Mak, Purnima Parthasarathy, Shirin Kalimuddin, Yi Hao Chan, Raymond T. P. Lin, Li Wei Ang, David C. Lye, Cheryl Sy Heng, Bernett Lee, Surinder Pada, Yee Sin Leo, Lisa F. P. Ng, Danielle E. Anderson, Louisa Sun, Rachael Pung, Sebastian Maurer-Stroh, Vernon J. Lee, Barnaby Edward Young, Paul A. Tambyah, Laurent Rénia, Gavin J. D. Smith, Seow Yen Tan, Wycliffe E. Wei |
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| Přispěvatelé: | Lee Kong Chian School of Medicine (LKCMedicine), National Centre for Infectious Diseases, Tan Tock Seng Hospital, National University of Singapore |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Adult Male Clade Medicine (General) Comorbidity Biology Systemic inflammation Logistic regression General Biochemistry Genetics and Molecular Biology Severity 03 medical and health sciences R5-920 0302 clinical medicine medicine Humans Transmission Medicine [Science] Hypoxia Aged Singapore SARS-CoV-2 Age Factors COVID-19 General Medicine Odds ratio Middle Aged Viral Load D614G Confidence interval 030104 developmental biology 030220 oncology & carcinogenesis Immunology Etiology Medicine Female medicine.symptom Viral load Cohort study Research Paper |
| Zdroj: | EBioMedicine EBIOMEDICINE EBioMedicine, Vol 66, Iss, Pp 103319-(2021) |
| ISSN: | 2352-3964 |
| Popis: | Background: Host determinants of severe coronavirus disease 2019 include advanced age, comorbidities and male sex. Virologic factors may also be important in determining clinical outcome and transmission rates, but limited patient-level data is available. Methods: We conducted an observational cohort study at seven public hospitals in Singapore. Clinical and laboratory data were collected and compared between individuals infected with different SARS-CoV-2 clades. Firth's logistic regression was used to examine the association between SARS-CoV-2 clade and development of hypoxia, and quasi-Poisson regression to compare transmission rates. Plasma samples were tested for immune mediator levels and the kinetics of viral replication in cell culture were compared. Findings: 319 patients with PCR-confirmed SARS-CoV-2 infection had clinical and virologic data available for analysis. 29 (9%) were infected with clade S, 90 (28%) with clade L/V, 96 (30%) with clade G (containing D614G variant), and 104 (33%) with other clades ‘O’ were assigned to lineage B.6. After adjusting for age and other covariates, infections with clade S (adjusted odds ratio (aOR) 0·030 (95% confidence intervals (CI): 0·0002–0·29)) or clade O (B·6) (aOR 0·26 (95% CI 0·064–0·93)) were associated with lower odds of developing hypoxia requiring supplemental oxygen compared with clade L/V. Patients infected with clade L/V had more pronounced systemic inflammation with higher concentrations of pro-inflammatory cytokines, chemokines and growth factors. No significant difference in the severity of clade G infections was observed (aOR 0·95 (95% CI: 0·35–2·52). Though viral loads were significantly higher, there was no evidence of increased transmissibility of clade G, and replicative fitness in cell culture was similar for all clades. Interpretation: Infection with clades L/V was associated with increased severity and more systemic release of pro-inflammatory cytokines. Infection with clade G was not associated with changes in severity, and despite higher viral loads there was no evidence of increased transmissibility. Published version |
| Databáze: | OpenAIRE |
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