GHEP-ISFG collaborative exercise on mixture profiles of autosomal STRs (GHEP-MIX01, GHEP-MIX02 and GHEP-MIX03): results and evaluation
| Autor: | Victor G. Saragoni, Fernando Rendo, Cíntia Alves, S. Pedrosa, F. Moreno, P. Madero, Elena Lopez de Santamaria, G. Plaza, M. Aler, Pedro A. Barrio, A. Hernández, Teresa Ribeiro, Jorge Puente, M.A. García, Federica Alessandrini, A. Ibarra, M.J. Jiménez, A. Sala, M. V. Masciovecchio, A. Gaviria, Juan A. Luque, Amaya Gorostiza, A. Gladys, R.M. Barretto, Nidia M. Modesti, A. Bofarull, S. Pagano, M. Crespillo, Begoña Martínez-Jarreta, L. Hombreiro, Sandra Maria Fonseca da Costa, E. Prat, Martin R. Whittle, G.M. Luque, M. Herrera, L. Andrade, Oscar Garcia |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
CIENCIAS MÉDICAS Y DE LA SALUD
General interest Computer science Applied psychology Criminal case Pathology and Forensic Medicine Task (project management) 03 medical and health sciences 0302 clinical medicine Complete information Surveys and Questionnaires Autosomal STRs Genetics Humans 030216 legal & forensic medicine Set (psychology) Simulation 030304 developmental biology 0303 health sciences Mixture interpretation Continuing education Spanish and Portuguese-Speaking Working Group Otras Ciencias Médicas General Vision Collaborative exercise DNA analysis Value (mathematics) Medicina Forense Microsatellite Repeats |
| Popis: | One of the main objectives of the Spanish and Portuguese-Speaking Group of the International Society for Forensic Genetics (GHEP-ISFG) is to promote and contribute to the development and dissemination of scientific knowledge in the area of forensic genetics. Due to this fact, GHEP-ISFG holds different working commissions that are set up to develop activities in scientific aspects of general interest. One of them, the Mixture Commission of GHEP-ISFG, has organized annually, since 2009, a collaborative exercise on analysis and interpretation of autosomal short tandem repeat (STR) mixture profiles. Until now, three exercises have been organized (GHEP-MIX01, GHEP-MIX02 and GHEP-MIX03), with 32, 24 and 17 participant laboratories respectively. The exercise aims to give a general vision by addressing, through the proposal of mock cases, aspects related to the edition of mixture profiles and the statistical treatment. The main conclusions obtained from these exercises may be summarized as follows. Firstly, the data show an increased tendency of the laboratories toward validation of DNA mixture profiles analysis following international recommendations (ISO/IEC 17025:2005). Secondly, the majority of discrepancies are mainly encountered in stutters positions (53.4%, 96.0% and 74.9%, respectively for the three editions). On the other hand, the results submitted reveal the importance of performing duplicate analysis by using different kits in order to reduce errors as much as possible. Regarding the statistical aspect (GHEP-MIX02 and 03), all participants employed the likelihood ratio (LR) parameter to evaluate the statistical compatibility and the formulas employed were quite similar. When the hypotheses to evaluate the LR value were locked by the coordinators (GHEP-MIX02) the results revealed a minor number of discrepancies that were mainly due to clerical reasons. However, the GHEP-MIX03 exercise allowed the participants to freely come up with their own hypotheses to calculate the LR value. In this situation the laboratories reported several options to explain the mock cases proposed and therefore significant differences between the final LR values were obtained. Complete information concerning the background of the criminal case is a critical aspect in order to select the adequate hypotheses to calculate the LR value. Although this should be a task for the judicial court to decide, it is important for the expert to account for the different possibilities and scenarios, and also offer this expertise to the judge. In addition, continuing education in the analysis and interpretation of mixture DNA profiles may also be a priority for the vast majority of forensic laboratories. Fil: Sala, Adriana Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Servicio de Huellas Digitales Genéticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Crespillo, M.. Instituto Nacional de Toxicología y Ciencias Forenses; España Fil: Barrio, P. A.. Instituto Nacional de Toxicología y Ciencias Forenses; España Fil: Luque, J. A.. Instituto Nacional de Toxicología y Ciencias Forenses; España Fil: Alves, Cíntia. Universidad de Porto; Portugal Fil: Aler, M.. Servicio de Laboratorio. Sección de Genética Forense y Criminalística; España Fil: Alessandrini, F.. Università Politecnica delle Marche. Department of Biomedical Sciences and Public Health; Italia Fil: Andrade, L.. Instituto Nacional de Medicina Legal e Ciências Forenses, Delegação do Centro. Serviço de Genética e Biologia Forenses; Portugal Fil: Barretto, R. M.. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil Fil: Bofarull, A.. Instituto Nacional de Toxicología y Ciencias Forenses; España Fil: Costa, S.. Instituto Nacional de Medicina Legal y Ciencias Forenses; Portugal Fil: García, M. A.. Servicio de Criminalística de la Guardia Civil. Laboratorio Central de Criminalística. Departamento de Biología; España Fil: García, O.. Basque Country Police. Forensic Genetics Section. Forensic Science Unit; España Fil: Gaviria, A.. Cruz Roja Ecuatoriana. Laboratorio de Genética Molecular; Ecuador Fil: Gladys, A.. Corte Suprema de Justicia de la Nación; Argentina Fil: Gorostiza, A.. Grupo Zeltia. Genomica S. A. U.. Laboratorio de Identificación Genética; España Fil: Hernández, A.. Instituto Nacional de Toxicología y Ciencias Forenses; España Fil: Herrera, M.. Laboratorio Genda S. A.; Argentina Fil: Hombreiro, L.. Jefatura Superior de Policía de Galicia. Brigada de Policía Científica. Laboratorio Territorial de Biología – ADN; España Fil: Ibarra, A. A.. Universidad de Antioquia; Colombia Fil: Jiménez, M. J.. Policia de la Generalitat – Mossos d’Esquadra. Divisió de Policia Científica. Àrea Central de Criminalística. Unitat Central de Laboratori Biològic; España Fil: Luque, G. M.. Instituto Nacional de Toxicología y Ciencias Forenses; España Fil: Madero, P.. Centro de Análisis Genéticos; España Fil: Martínez Jarreta, B.. Universidad de Zaragoza; España Fil: Masciovecchio, M. Verónica. IACA Laboratorios; Argentina Fil: Modesti, Nidia Maria. Provincia de Córdoba. Poder Judicial; Argentina Fil: Moreno, F.. Servicio Médico Legal. Unidad de Genética Forense; Chile Fil: Pagano, S.. Dirección Nacional de Policía Técnica. Laboratorio de Análisis de ADN para el CODIS; Uruguay Fil: Pedrosa, S.. Navarra de Servicios y Tecnologías S. A. U.; España Fil: Plaza, G.. Neodiagnostica S. L.; España Fil: Prat, E.. Comisaría General de Policía Científica. Laboratorio de ADN; España Fil: Puente, J.. Laboratorio de Genética Clínica S. L.; España Fil: Rendo, F.. Universidad del País Vasco; España Fil: Ribeiro, T.. Instituto Nacional de Medicina Legal e Ciências Forenses, Delegação Sul. Serviço de Genética e Biologia Forenses; Portugal Fil: Santamaría, E.. Instituto Nacional de Toxicología y Ciencias Forenses; España Fil: Saragoni, V. G.. Servicio Médico Legal. Departamento de Laboratorios. Unidad de Genética Forense; Chile Fil: Whittle, M. R.. Genomic Engenharia Molecular; Brasil |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|