Reverse Transcriptase Polymerase Chain Reaction for Detecting Ewing’s Sarcoma in Archival Fine Needle Aspiration Biopsies
| Autor: | Holger Nagel, Silke Reimer, Ruschenburg I, Thilo Schlott, Droese M |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Pathology
medicine.medical_specialty Histology Chromosomes Human Pair 22 Cytodiagnosis Bone Neoplasms Sarcoma Ewing Biology Polymerase Chain Reaction Translocation Genetic Pathology and Forensic Medicine law.invention 03 medical and health sciences 0302 clinical medicine law Proto-Oncogene Proteins medicine Humans RNA Messenger Polymerase chain reaction 030304 developmental biology 0303 health sciences Base Sequence medicine.diagnostic_test Proto-Oncogene Protein c-fli-1 Peripheral Primitive Neuroectodermal Tumor Chromosomes Human Pair 11 Biopsy Needle RNA Ewing's sarcoma General Medicine medicine.disease Reverse transcriptase 3. Good health DNA-Binding Proteins Fine-needle aspiration Cytopathology 030220 oncology & carcinogenesis Trans-Activators Sarcoma |
| Zdroj: | Acta Cytologica. 41:795-801 |
| ISSN: | 1938-2650 0001-5547 |
| DOI: | 10.1159/000332706 |
| Popis: | OBJECTIVE Previous studies on pediatric soft tissue sarcomas have demonstrated a chromosomal 11;22 (q24;q12) translocation in Ewing's sarcoma (ES) and peripheral primitive neuroectodermal tumors that appears to be a unique oncogenic marker. To investigate the usefulness of reverse transcriptase polymerase chain reaction (RT-PCR) as an ancillary method for cytodiagnosis, we tested archival aspirates derived from ES patients to establish whether any beta-actin RNA expression or tumor-specific EWS/FLI-1 gene translocation had occurred. STUDY DESIGN Sixteen skeletal specimens aspirated 10-15 years earlier from patients with cytologic and histologic diagnoses of ES were prepared for PCR. The amplification products were sequenced. RESULTS Amplifiable RNA was detected in smears from 12 patients by beta-actin RT-PCR. Seven aspirates from beta-actin-positive patients showed ES-specific genomic translocation (58%). Sequence analysis of the resulting PCR fragments revealed EWS/FLI-1 fusion transcriptions of varying length. CONCLUSION When RNA was retrievable, RT-PCR applied to routinely stained aspirate smears was a highly specific method in differential diagnosis of ES. |
| Databáze: | OpenAIRE |
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