RNase III Controls the Degradation of corA mRNA in Escherichia coli
| Autor: | Nam-Chul Ha, Younghoon Lee, Boram Lim, Se-Hoon Sim, Kyungsub Kim, Minji Sim, Kangseok Lee, Che Ok Jeon |
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| Rok vydání: | 2012 |
| Předmět: |
Ribonuclease III
Untranslated region RNA Stability RNase P Cleavage (embryo) medicine.disease_cause Microbiology Nickel Escherichia coli medicine Magnesium RNA Messenger Cation Transport Proteins Molecular Biology Messenger RNA Base Sequence biology Escherichia coli Proteins Biological Transport Cobalt Gene Expression Regulation Bacterial Articles Molecular biology RNase MRP Biochemistry Mutation biology.protein |
| Zdroj: | Journal of Bacteriology. 194:2214-2220 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.00099-12 |
| Popis: | In Escherichia coli , the corA gene encodes a transporter that mediates the influx of Co 2+ , Mg 2+ , and Ni 2+ into the cell. During the course of experiments aimed at identifying RNase III-dependent genes in E. coli , we observed that steady-state levels of corA mRNA as well as the degree of cobalt influx into the cell were dependent on cellular concentrations of RNase III. In addition, changes in corA expression levels by different cellular concentrations of RNase III were closely correlated with degrees of resistance of E. coli cells to Co 2+ and Ni 2+ . In vitro and in vivo cleavage analyses of corA mRNA identified RNase III cleavage sites in the 5′-untranslated region of the corA mRNA. The introduction of nucleotide substitutions at the identified RNase III cleavage sites abolished RNase III cleavage activity on corA mRNA and resulted in prolonged half-lives of the mRNA, which demonstrates that RNase III cleavage constitutes a rate-determining step for corA mRNA degradation. These findings reveal an RNase III-mediated regulatory pathway that functions to modulate corA expression and, in turn, the influx of metal ions transported by CorA in E. coli . |
| Databáze: | OpenAIRE |
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