Cytochrome P450 2C8*2 allele in Botswana: human genetic diversity and public health implications
| Autor: | Thato Motshoge, Charles Muthoga, Giacomo Maria Paganotti, R. Romano, Joel Allotey, Isaac K. Quaye, Leabaneng Tawe |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
parasitology Veterinary (miscellaneous) genotype polymerase chain reaction Ethnic group Bantu ethnic group Black People Human genetic variation Biology gene frequency cytochrome p-450 cyp2C8 infectious diseases ethnic groups polymorphism 03 medical and health sciences 0302 clinical medicine male Genotype Ethnicity 030212 general & internal medicine Allele humans Allele frequency pharmacogenetics Genetics botswana cyp2C8 san ethnic group adolescent african continental ancestry group alleles child cytochrome p-450 enzyme system female polymorphism genetic polymorphism restriction fragment length Polymorphism Genetic restriction fragment length 030104 developmental biology Insect Science Gene polymorphism Restriction fragment length polymorphism genetic Pharmacogenetics Polymorphism Restriction Fragment Length |
| Popis: | Human cytochrome P450 2C8 is a highly polymorphic gene and shows variation according to ethnicity. The CYP2C8*2 is a slow drug metabolism allele and shows 10-24% frequency in Black populations. The objective of this study was to assess the prevalence of CYP2C8*2 allele in Botswana among the San (or Bushmen) and the Bantu ethnic groups. For that purpose we recruited 544 children of the two ethnicities in three districts of Botswana from primary schools, collected blood samples, extracted DNA and genotyped them through PCR-based restriction fragment length polymorphism analysis. The results demonstrated that in the San the prevalence of the CYP2C8*2 allele is significantly higher than among the Bantu-related ethnic groups (17.5% and 8.5% for San and Bantu, respectively; P=0.00002). These findings support the evidence of a different genetic background of the San with respect to Bantu-related populations, and highlight a possible higher risk of longer drug clearance or poor level of activation of pro-drugs among the San group. |
| Databáze: | OpenAIRE |
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