Near-infrared fluorescence imaging of mouse myocardial microvascular endothelium using Cy5.5-lectin conjugate
| Autor: | V.V. Kupriyanov, Olga Jilkina, Lori Gregorash, Saro Bascaramurty, Bozena Kuzio, Cecilia Nguyen |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Near-Infrared Fluorescence Imaging Pathology medicine.medical_specialty Endothelium cardiac imaging techniques General Physics and Astronomy Spleen Lycopersicon esculentum lectin fluorescence microscopy General Biochemistry Genetics and Molecular Biology Mice medicine Fluorescence microscope Animals General Materials Science mouse Fluorescent Dyes Kidney biology Optical Imaging General Engineering Lectin General Chemistry Carbocyanines Cy5.5 Coronary Vessels Molecular biology endothelial cells Staining Mice Inbred C57BL Perfusion medicine.anatomical_structure Microvessels biology.protein Female Endothelium Vascular Plant Lectins Conjugate |
| Zdroj: | Journal of Biophotonics. 5:754-767 |
| ISSN: | 1864-063X |
| Popis: | Cy5.5-lectin, a non-toxic conjugate, combines the benefits of near-infrared (NIR) imaging, such as significant reduction of background fluorescence and increased tissue depth penetration, with its affinity for vascular endothelial cells. When compared to endothelial staining methods using FITC-lectin and ICAM2 antibodies, Cy5.5-lectin was confirmed to specifically bind endothelial cells and produce a fluorescence signal both in real-time and post-infusion. Ex-vivo experiments with isolated hearts demonstrated that binding was limited to perfused areas of the myocardium. With mouse in-vivo tail-vein injections, other organs such as the liver, spleen, and kidney were also stained and yielded similar quality images of the heart. |
| Databáze: | OpenAIRE |
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