Hop bitter acids inhibit tumorigenicity of hepatocellular carcinoma cells in vitro
| Autor: | B Czech, M Saugspier, Manfred Gehrig, Christoph Dorn, Claus Hellerbrand, Jörg Heilmann |
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| Rok vydání: | 2012 |
| Předmět: |
Cancer Research
Carcinoma Hepatocellular Humulus lupulus Aspartate transaminase Pharmacology Hop (networking) Downregulation and upregulation Cell Movement Cyclohexenes Tumor Cells Cultured Humans Aspartate Aminotransferases Phosphorylation Humulus Cell Proliferation Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Dose-Response Relationship Drug biology Oncogene Cyclohexanones Plant Extracts Terpenes Liver Neoplasms NF-kappa B Biological activity General Medicine biology.organism_classification Antineoplastic Agents Phytogenic In vitro Transcription Factor AP-1 Oncology Apoptosis biology.protein Cancer research Drug Screening Assays Antitumor Acids |
| Zdroj: | Oncology Reports. 28:1423-1428 |
| ISSN: | 1791-2431 1021-335X |
| DOI: | 10.3892/or.2012.1925 |
| Popis: | Bitter acids (BAs) from the hop plant Humulus lupulus L. exhibit multiple beneficial biological properties with promising effects in cancer therapy and prevention, but information regarding the effects on hepatocellular carcinoma (HCC) is missing. Here, we used two different hop bitter acid extracts enriched for either α-acids or β-acids to obtain insight into whether biological activity varies between these two groups of BAs. At a concentration of 25 µg/ml, only the β-acid rich started to induce aspartate transaminase (AST) release, and a significant increase was detected with 50 µg/ml of both extracts. Already at lower concentrations both extracts led to a dose-dependent inhibition of proliferation, and migration was suppressed at a concentration as low as 5 µg/ml in HCC cells. The focus on different signaling pathways revealed an inhibition of ERK1/2 phosphorylation, downregulation of AP-1 activity and an alleviation of nuclear factor κB (NFκB) activity in HepG2 cells incubated with 5 µg/ml of both extracts, whereby the β-acid rich extract showed more pronounced effects. In conclusion, we identified ERK1/2, AP-1 and NFκB, which are important factors in tumor development and progression, as targets of hop BAs. Thus, these data suggest the potential use of BAs as functional nutrients for both prevention and treatment of HCC. |
| Databáze: | OpenAIRE |
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