Development and Validation of a Fully Automated Platform for Extended Blood Group Genotyping
| Autor: | Stephanie A. Boccoz, Céline A. Mandon, Loïc J. Blum, Christophe A. Marquette, Benjamin P. Corgier, Gaelle C. Le Goff |
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| Přispěvatelé: | Génie Enzymatique, Membrane Biomimétique et Assemblages Supramoléculaires (GEMBAS), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS), AXO Science (Axo S) |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Erythrocytes Blood transfusion Genotyping Techniques medicine.medical_treatment 030204 cardiovascular system & hematology Biology Polymorphism Single Nucleotide Pathology and Forensic Medicine Serology 03 medical and health sciences 0302 clinical medicine Genotype Multiplex polymerase chain reaction medicine Humans Typing [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] Genotyping Oligonucleotide Array Sequence Analysis [CHIM.ORGA]Chemical Sciences/Organic chemistry Molecular biology 3. Good health Red blood cell 030104 developmental biology medicine.anatomical_structure Blood Grouping and Crossmatching Fully automated Blood Group Antigens Molecular Medicine Multiplex Polymerase Chain Reaction |
| Zdroj: | Journal of Molecular Diagnostics Journal of Molecular Diagnostics, American Society for Investigative Pathology (ASIP), 2016, 18 (1), pp.144-152. ⟨10.1016/j.jmoldx.2015.09.002⟩ |
| ISSN: | 1525-1578 |
| DOI: | 10.1016/j.jmoldx.2015.09.002 |
| Popis: | International audience; Thirty-five blood group systems, containing >300 antigens, are listed by the International Society of Blood Transfusion. Most of these antigens result from a single nucleotide polymorphism. Blood group typing is conventionally performed by serology. However, this technique has some limitations and cannot respond to the growing demand of blood products typed for a large number of antigens. The knowledge of the molecular basis of these red blood cell systems allowed the implementation of molecular biology methods in immunohematology laboratories. Here, we describe a blood group genotyping assay based on the use of TKL immobilization support and microarray-based HIFI technology that takes approximately 4 hours and 30 minutes from whole-blood samples to results analysis. Targets amplified by multiplex PCR were hybridized on the chip, and a revelation step allowed the simultaneous identification of up to 24 blood group antigens, leading to the determination of extended genotypes. Two panels of multiplex PCR were developed: Panel 1 (KEL1/2, KEL3/4; JK1/2; FY1/2; MNS1/2, MNS3/4, FY*Fy et FY*X) and Panel 2 (YT1/2; CO1/2; DO1/2, HY+, Jo(a+); LU1/2; DI1/2). We present the results of the evaluation of our platform on a panel of 583 and 190 blood donor samples for Panel 1 and 2, respectively. Good correlations (99% to 100%) with reference were obtained. |
| Databáze: | OpenAIRE |
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