Interleukin-6 Production in Human Intestinal Epithelial Cells Increases in Association with the Heat Shock Response
| Autor: | Per-Olof Hasselgren, M. Ryan Moon, Andrew L. Salzman, Christine D. Kane, Josef E. Fischer, Alexander A. Parikh |
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| Rok vydání: | 1998 |
| Předmět: |
Hyperthermia
Hot Temperature Transcription Genetic Enterocyte Inflammation Biology Polymerase Chain Reaction Heat shock protein medicine Humans HSP70 Heat-Shock Proteins RNA Messenger Intestinal Mucosa Heat shock Messenger RNA Interleukin-6 medicine.disease Molecular biology Blot medicine.anatomical_structure Shock (circulatory) Surgery Caco-2 Cells medicine.symptom Heat-Shock Response Interleukin-1 |
| Zdroj: | Journal of Surgical Research. 77:40-44 |
| ISSN: | 0022-4804 |
| DOI: | 10.1006/jsre.1998.5332 |
| Popis: | Background.In recent studies, IL-1β stimulated the production of IL-6 in human enterocytes. The heat shock response influences the production of inflammatory mediators in certain cell types. We tested the hypothesis that heat shock regulates IL-1β-induced IL-6 production in human intestinal epithelial cells. Materials and methods.Cultured Caco-2 cells, a human intestinal epithelial cell line, were exposed to thermal heat shock at 43°C for 1 h and recovered at 37°C for 1 h. Cells were harvested for analysis of heat shock protein-70 (HSP-70) production by Western blotting. In other experiments, IL-1β (0.5 ng/ml) was added following heat shock and recovery. IL-6 protein was measured in culture medium after 24 h by enzyme-linked immunosorbent assay and IL-6 messenger RNA (mRNA) levels were measured after 4 h by competitive reverse transcriptase polymerase chain reaction. Results.Heat shock resulted in the production of HSP-70 and potentiated IL-1β-induced IL-6 production. The response to heat shock was associated with increased IL-6 mRNA levels. Conclusions.The results suggest that IL-1β-induced IL-6 production in human enterocytes is increased in association with the heat shock response. The biological role of heat shock-potentiated IL-6 production in the enterocyte remains to be determined. |
| Databáze: | OpenAIRE |
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