Sensitive analytical methods for 22 relevant insecticides of 3 chemical families in honey by GC-MS/MS and LC-MS/MS

Autor: Delphine Paradis, Luc P. Belzunces, Jean-Marc Bonmatin, Géraldine Bérail
Přispěvatelé: Laboratoire de l'environnement et de l'alimentation de la Vendée, Centre de biophysique moléculaire (CBM), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université d'Orléans (UO), Laboratoire de Toxicologie Environnementale (UR 406 Abeilles et Environnement), Institut National de la Recherche Agronomique (INRA), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Abeilles & Environnement (UR 406 ), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Conseil General de la Vendee, ANRT (Association Nationale de la Recherche et de la Technologie, France)
Rok vydání: 2013
Předmět:
Insecticides
multi-residue method
Pyridines
[SDV]Life Sciences [q-bio]
pesticide-residue
010501 environmental sciences
Tandem mass spectrometry
01 natural sciences
Biochemistry
MESH: Bees
Analytical Chemistry
Matrix (chemical analysis)
Limit of Detection
Tandem Mass Spectrometry
Pyrethrins
multiresidue determination
MESH: Animals
Chemistry
MESH: Chromatography
Gas
Honey
Bees
gas-chromatography
Calibration
apis-mellifera l
MESH: Honey
QuEChERS
Chromatography
Gas
Apiary
MESH: Limit of Detection
Context (language use)
insecticide residues
Quechers
MESH: Calibration
MESH: Pyrethrins
pyrethroids
[CHIM.ANAL]Chemical Sciences/Analytical chemistry
Animals
solid-phase extraction
liquid-liquid microextraction
electron-capture
0105 earth and related environmental sciences
Detection limit
Chromatography
MESH: Pyridines
010401 analytical chemistry
MESH: Tandem Mass Spectrometry
mass-spectrometric detection
MESH: Insecticides
0104 chemical sciences
neonicotinoid insecticide
Pyrazoles
neonicotinoids
Gas chromatography
Gas chromatography–mass spectrometry
MESH: Chromatography
Liquid
MESH: Pyrazoles
Chromatography
Liquid
Zdroj: Analytical and Bioanalytical Chemistry
Analytical and Bioanalytical Chemistry, Springer Verlag, 2014, 406 (2), pp.621-633
Analytical and Bioanalytical Chemistry, Springer Verlag, 2014, 406 (2), pp.621-633. ⟨10.1007/s00216-013-7483-z⟩
ISSN: 1618-2650
1618-2642
DOI: 10.1007/s00216-013-7483-z
Popis: International audience; Several methods for analyzing pesticides in honey have been developed. However, they do not always reach the sufficiently low limits of quantification (LOQ) needed to quantify pesticides toxic to honey bees at low doses. To properly evaluate the toxicity of pesticides, LOQ have to reach at least 1 ng/g. In this context, we developed extraction and analytical methods for the simultaneous detection of 22 relevant insecticides belonging to three chemical families (neonicotinoids, pyrethroids, and pyrazoles) in honey. The insecticides were extracted with the QuEChERS method that consists in an extraction and a purification with mixtures of salts adapted to the matrix and the substances to be extracted. Analyses were performed by gas chromatography coupled with tandem mass spectrometry (GC-MS/MS) for the pyrazoles and the pyrethroids and by high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) for the neonicotinoids and ethiprole. Calibration curves were built from various honey types fortified at different concentrations. Linear responses were obtained between 0.2 and 5 ng/g. Limits of detection (LOD) ranged between 0.07 and 0.2 ng/g, and LOQ ranged between 0.2 and 0.5 ng/g. The mean extraction yields ranged between 63 % and 139 % with RSD
Databáze: OpenAIRE
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