Chelating Langmuir-Blodgett film: a new versatile chemiluminescent sensing layer for biosensor applications
| Autor: | Agnès Girard-Egrot, Christophe A. Marquette, Aurélie A.-M. Santafé, Loïc J. Blum |
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| Přispěvatelé: | Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École Supérieure Chimie Physique Électronique de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Depierre, Frédérique |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Surface Properties
Oleic Acids 02 engineering and technology Biosensing Techniques 010402 general chemistry 01 natural sciences Langmuir–Blodgett film Catalysis law.invention chemistry.chemical_compound law Nickel Monolayer Electrochemistry Imidazole Organic chemistry Nanotechnology General Materials Science Chelation [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] Spectroscopy Chemiluminescence Chelating Agents chemistry.chemical_classification Ligand Biomolecule Lysine Water Succinates Surfaces and Interfaces 021001 nanoscience & nanotechnology Condensed Matter Physics Lipid Metabolism Combinatorial chemistry 0104 chemical sciences Immobilized Proteins chemistry Luminescent Measurements Luminol 0210 nano-technology Biosensor Copper Histamine |
| Zdroj: | Langmuir Langmuir, American Chemical Society, 2010, 26 (3), pp.2160-66 |
| ISSN: | 0743-7463 1520-5827 |
| Popis: | International audience; The present study reports the achievement of a new chemiluminescent sensing layer able to simultaneously (i) play an active role on ligand immobilization and (ii) serve as a catalyst in detection processes for label-free biosensor applications. This new type of active Langmuir−Blodgett (LB) monolayer has been designed by using a chelating lipid (Ni-NTA-DOGS). Thanks to the chelated metallic cation, this peculiar lipid exhibits luminol chemiluminescence catalysis properties in the presence of hydrogen peroxide. Upon biomolecule interaction through imidazole ring chelation (mediated by the metallic cation bound to the lipid headgroup), the chemiluminescent signal can be modulated. The first chemiluminescent signal acquisition experiments have shown a strong and homogeneous signal of the chelating layer. Upon histamine interactions, a histidine derivative used as a marker of fresh food quality, we succeeded in obtaining as a proof of concept a chemiluminescent signal variation without any derivatization of the target molecule. This signal variation was shown to be directly correlated to the histamine concentration with a limit of detection of 2 μg/mL. |
| Databáze: | OpenAIRE |
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