Direct lentivirus injection for fast and efficient gene transfer into brown and beige adipose tissue
| Autor: | Katarina Klepac, Linda S. Hoffmann, Aileen Balkow, Thorsten Gnad, Katrin Zimmermann, Anja Glöde, Alexander Pfeifer |
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| Rok vydání: | 2016 |
| Předmět: |
medicine.medical_specialty
biology beige adipose tissue Transgene Adipose tissue brown adipose tissue White adipose tissue biology.organism_classification medicine.disease Fat pad medicine.anatomical_structure Endocrinology lentivirus In vivo Internal medicine Lentivirus Brown adipose tissue Protocol medicine General Earth and Planetary Sciences gene transfer Infiltration (medical) General Environmental Science |
| Zdroj: | Journal of Biological Methods |
| ISSN: | 2326-9901 |
| Popis: | Brown adipose tissue is a special type of fat contributing to energy expenditure in human newborns and adults. Moreover, subcutaneous white adipose tissue has a high capacity to adapt an energy-consuming, brown-like/beige phenotype. Here, we developed an easy to handle and fast to accomplish method to efficiently transfer genes into brown and beige fat pads in vivo. Lentiviral vectors are directly injected into the target fat pad of anaesthetized mice through a small incision using a modified, small needle connected to a microsyringe, which is well suited for infiltration of adipose tissues. Expression of the target gene can be detected in brown/beige fat one week after injection. The method can be applied within minutes to efficiently deliver transgenes into subcutaneous adipose tissues. Thus, this protocol allows for studying genes of interest in a timely manner in murine brown/beige fat and could potentially lead to new gene therapies for obesity. Brown adipose tissue is a special type of fat contributing to energy expenditure in human newborns and adults. Moreover, subcutaneous white adipose tissue has a high capacity to adapt an energy-consuming, brown-like/beige phenotype. Here, we developed an easy to handle and fast to accomplish method to efficiently transfer genes into brown and beige fat pads in vivo. Lentiviral vectors are directly injected into the target fat pad of anaesthetized mice through a small incision using a modified, small needle connected to a microsyringe, which is well suited for infiltration of adipose tissues. Expression of the target gene can be detected in brown/beige fat one week after injection. The method can be applied within minutes to efficiently deliver transgenes into subcutaneous adipose tissues. Thus, this protocol allows for studying genes of interest in a timely manner in murine brown/beige fat and could potentially lead to new gene therapies for obesity. |
| Databáze: | OpenAIRE |
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