Cytotoxicity, Antiproliferative Effects, and Apoptosis Induction of Methanolic Extract of Cynometra cauliflora Linn. Whole Fruit on Human Promyelocytic Leukemia HL-60 Cells
Autor: | Abdul Manaf Ali, Afnani Alwi, Nashriyah Mat, T-Johari S. A. Tajudin, Abu Bakar Siti-Aishah, A. Aziz M. Yusran |
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Jazyk: | angličtina |
Rok vydání: | 2012 |
Předmět: |
biology
Article Subject Cell growth business.industry lcsh:Other systems of medicine biology.organism_classification lcsh:RZ201-999 Molecular biology 3T3 cells Cynometra cauliflora medicine.anatomical_structure Complementary and alternative medicine Cell culture Apoptosis Immunology medicine Cytotoxic T cell MTT assay Cytotoxicity business Research Article |
Zdroj: | Evidence-based Complementary and Alternative Medicine : eCAM Evidence-Based Complementary and Alternative Medicine, Vol 2012 (2012) |
ISSN: | 1741-4288 1741-427X |
Popis: | Methanolic extract ofCynometra cauliflorawhole fruit was assayed for cytotoxicity against the human promyelocytic leukemia HL-60 and the normal mouse fibroblast NIH/3T3 cell lines by using the MTT assay. The CD50of the extract for 72 hours was 0.9 μg/mL whereas the value for the cytotoxic drug vincristine was 0.2 μg/mL. The viability of the NIH/3T3 cells was at 80.0% when treated at 15.0 μg/mL. The extract inhibited HL-60 cell proliferation with dose dependence. AO/PI staining of HL-60 cells treated with the extract revealed that majority of cells were in the apoptotic cell death mode. Flow cytometry analysis of HL-60 cells treated at CD50of the extract showed that the early apoptotic cells were 31.0, 26.3 and 19.9% at 24, 48, and 72 hours treatment, respectively. The percentage of late apoptotic cells was increased from 62.0 at 24 hours to 64.1 and 70.2 at 48 and 72 hours, respectively. Meanwhile, percent of necrotic cells were 4.9, 6.6, and 8.5 at 24, 48, and 72 hours, respectively. This study has shown that the methanolic extract ofC. cauliflorawhole fruit was cytotoxic towards HL-60 cells and induced the cells into apoptotic cell death mode, but less cytotoxic towards NIH/3T3 cells. |
Databáze: | OpenAIRE |
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