Adipose Tissue Expression of PACAP, VIP, and Their Receptors in Response to Cold Stress

Autor: Maeghan A. M. Forster, Daemon L. Cline, Sarah L. Gray, Landon I. Short
Rok vydání: 2018
Předmět:
Male
0301 basic medicine
medicine.medical_specialty
RNA Splicing
Adipose tissue
White adipose tissue
Biology
PACAP
Article
Mice
03 medical and health sciences
Cellular and Molecular Neuroscience
chemistry.chemical_compound
0302 clinical medicine
Adipose Tissue
Brown
Internal medicine
Adipocyte
Brown adipose tissue
medicine
Cold acclimation
Animals
Receptor
Cells
Cultured
Cold-Shock Response
mRNA expression
Adipose tissues
Thermogenesis
General Medicine
Mice
Inbred C57BL
VIP
Lipid metabolism
030104 developmental biology
medicine.anatomical_structure
Endocrinology
chemistry
Basal metabolic rate
Pituitary Adenylate Cyclase-Activating Polypeptide
Receptors
Vasoactive Intestinal Peptide
hormones
hormone substitutes
and hormone antagonists
030217 neurology & neurosurgery
Receptors
Pituitary Adenylate Cyclase-Activating Polypeptide
Type I
Vasoactive Intestinal Peptide
Zdroj: Journal of Molecular Neuroscience
ISSN: 1559-1166
0895-8696
DOI: 10.1007/s12031-018-1099-x
Popis: Obesity arises from disrupted energy balance and is caused by chronically higher energy intake compared to expenditure via basal metabolic rate, exercise, and thermogenesis. The brown adipose tissue (BAT), the primary thermogenic organ, has received considerable attention as a potential therapeutic target due to its ability to burn lipids in the production of heat. Pituitary adenylate cyclase-activating polypeptide (PACAP) has been identified as a key regulator of the physiological stress response both centrally and peripherally. While PACAP has been shown to increase thermogenesis by acting at the hypothalamus to increase sympathetic output to BAT, a peripheral role for PACAP-activated thermogenesis has not been studied. We identified PACAP receptor (PAC1, VPAC1/2) expression for the first time in murine BAT and confirmed their expression in white adipose tissues. PAC1 receptor expression was significantly altered in all three adipose tissues studied in response to 3.5-week cold acclimation, with expression patterns differing by depot type. In primary cell culture, VPAC1 was increased in differentiated compared to non-differentiated brown adipocytes, and the same trend was observed for the PACAP-specific receptor PAC1 in gonadal white fat primary cultures. The primary PAC1R mRNA splice variant in interscapular BAT was determined as isoform 2 by RNA-Seq. These results show that PACAP receptors are present in adipose tissues and may have important functional roles in adipocyte differentiation, lipid metabolism, or adipose sensitization to sympathetic signaling in response to thermogenic stimuli.
Databáze: OpenAIRE
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