Neisseria gonorrhoeaeThat Infect Men Have Lipooligosaccharides with Terminal N-Acetyllactosamine Repeats
| Autor: | H Schneider, J M Griffiss, Constance M. John |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Lipopolysaccharides
Male Glycoside Hydrolases medicine.drug_class Electrospray ionization Molecular Sequence Data Monoclonal antibody Mass spectrometry medicine.disease_cause Biochemistry Microbiology chemistry.chemical_compound medicine Humans Hexose Molecular Biology chemistry.chemical_classification Amino Sugars Cell Biology Oligosaccharide Neisseria gonorrhoeae N-Acetyllactosamine Matrix-assisted laser desorption/ionization Carbohydrate Sequence chemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization |
| Zdroj: | Journal of Biological Chemistry. 274:1017-1025 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.274.2.1017 |
| Popis: | Infectious Neisseria gonorrhoeae make relatively large lipooligosaccharides (LOS) that structurally resemble human glycosphingolipids. MS11mkC is an LOS variant of N. gonorrhoeae strain MS11 which was isolated from men at the onset of dysuria (Schneider, H., Griffiss, J. M., Boslego, J. W., Hitchcock, P. J., Zahos, K. M., and Apicella, M. A. (1991) J. Exp. Med. 174, 1601-1605). Delayed extraction matrix-assisted laser desorption and ionization and electrospray ionization mass spectrometry of O-deacylated MS11mkC LOS produced ions consistent with known LOS which have lacto-N-neotetraose (Galbeta1--4GlcNAcbeta1--3Galbeta1--4Glc; paraglobosyl; monoclonal antibodies (mAbs) 1B2(+) and 06B4(+)) and GalNAc--lacto-N-neotetraose (gangliosyl; mAb 1-1-M+) oligosaccharides. Ion peaks for a larger LOS which also bound mAb 1B2 indicated the addition of a hexose (+162 Da) to gangliosyl LOS or the addition of a hexose and a N-acetylhexosamine (+365 Da) to paraglobosyl LOS. Analysis of HF-treated and O-deacylated LOS revealed three major components present in a phosphoethanolamine (PEA)0 and a PEA1 series. Digestion of MS11mkC LOS by beta-N-acetylhexosaminidase and beta-galactosidase, alone and sequentially, combined with mAb binding patterns, confirmed the presence of a nonreducing terminal repeating LacNAc ((Galbeta1--4GlcNAc)2) on the largest LOS, rather than a parallel oligosaccharide structure. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|