New Role of P. brasiliensis α-Glucan: Differentiation of Non-conventional Dendritic Cells
| Autor: | Claire da Silva Santos, Anamélia Lorenzetti Bocca, Cláudia Brodskyn, Clara Luna Marina, Cecília Favali, Ana Camila Oliveira Souza, Natalia Machado Tavares, Naiara Caroline Soares, Paulo Henrique de Holanda Veloso Júnior, Márcio Sousa Jerônimo |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Microbiology (medical)
dendritic cell differentiation Cell lcsh:QR1-502 Dendritic cell differentiation Paracoccidiodomycosis Microbiology lcsh:Microbiology Proinflammatory cytokine Cell wall 03 medical and health sciences Immune system medicine 030304 developmental biology Paracoccidioides brasiliensis 0303 health sciences biology 030306 microbiology Chemistry cell wall fractions glucan Dendritic cell biology.organism_classification Cell biology medicine.anatomical_structure CD80 |
| Zdroj: | Frontiers in Microbiology, Vol 10 (2019) |
| ISSN: | 1664-302X |
| DOI: | 10.3389/fmicb.2019.02445 |
| Popis: | The cell wall has a critical role in the host immune response to fungal pathogens. In this study, we investigated the influence of two cell wall fractions of the dimorphic fungi Paracoccidioides brasiliensis (Pb) in the in vitro generation of monocyte-derived dendritic cells (MoDCs). Monocytes were purified from the peripheral blood of healthy donors and cultivated for 7 days in medium supplemented with IL-4 and GM-CSF in the presence of Pb cell wall fractions: the alkali-insoluble F1, constituted by β-1,3-glucans, chitin and proteins, and the alkali-soluble F2, mainly constituted by α-glucan. MoDCs phenotypes were evaluated regarding cell surface expression of CD1a, DC-SIGN, HLA-DR, CD80, and CD83 and production of cytokines. The α-glucan-rich cell wall fraction downregulated the differentiation of CD1a+ MoDCs, a dendritic cell subset that stimulate Th1 responses. The presence of both cell fractions inhibited DC-SIGN and HLA-DR expression, while the expression of maturation markers was differentially induced in CD1a– MoDCs. Differentiation upon F1 and F2 stimulation induced mixed profile of inflammatory cytokines. Altogether, these data demonstrate that Pb cell wall fractions differentially induce a dysregulation in DCs differentiation. Moreover, our results suggest that cell wall α-glucan promote the differentiation of CD1a– DCs, potentially favoring Th2 polarization and contributing to pathogen persistence. |
| Databáze: | OpenAIRE |
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