Isolation of anticancer constituents from Cucumis prophetarum var. prophetarum through bioassay-guided fractionation
| Autor: | Mahmoud S. Ahmed, Hesham S. M. Soliman, Lucas C. Kopel, Fathi T. Halaweish, Abdulrhman Alsayari, Sivakumar Annadurai |
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| Rok vydání: | 2018 |
| Předmět: |
Ethyl acetate
Fractionation Chemical Fractionation 01 natural sciences Cucumis 03 medical and health sciences chemistry.chemical_compound Breast cancer 0302 clinical medicine Cucurbitacins Cucumis prophetarum Cell Line Tumor Humans Structure–activity relationship Preliminary SAR Cytotoxicity Traditional medicine Plant Extracts 010405 organic chemistry Chemistry Cucurbitacin Cucumis prophetarum var. prophetarum lcsh:Other systems of medicine General Medicine lcsh:RZ201-999 Antineoplastic Agents Phytogenic 0104 chemical sciences Anticancer Complementary and alternative medicine Cell culture Bioassay-guided fractionation 030220 oncology & carcinogenesis Biological Assay Research Article |
| Zdroj: | BMC Complementary and Alternative Medicine, Vol 18, Iss 1, Pp 1-12 (2018) BMC Complementary and Alternative Medicine |
| ISSN: | 1472-6882 |
| DOI: | 10.1186/s12906-018-2295-5 |
| Popis: | Background Cucumis prophetarum var. prophetarum is used in Saudi folk medicine for treating liver disorders and grows widely between Abha and Khamis Mushait City, Saudi Arabia. Methods Bioassay-guided fractionation and purification were used to isolate the main active constituents of Cucumis prophetarum var. prophetarum fruits. These compounds were structurally elucidated using NMR spectroscopy, mass spectral analyses and x-ray crystallography. All fractions, sub-fractions and pure compounds were screened for their anticancer activity against six cancer cell lines. Results The greatest cytotoxic activity was found to be in the ethyl acetate fraction, resulting in the isolation of five cucurbitacin compounds [E, B, D, F-25 acetate and Hexanorcucurbitacin D]. Among the cucurbitacins that were isolated and tested cucurbitacin B and E showed potent cytotoxicity activities against all six human cancer cell lines. Conclusion Human breast cancer cell lines were found to be the most sensitive to cucurbitacins. Preliminary structure activity relationship (SAR) for cytotoxic activity of Cucurbitacins against human breast cancer cell line MDA-MB-231 has been reported. |
| Databáze: | OpenAIRE |
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